BibTex format
@article{Bonnin:2024:10.1016/S2666-5247(23)00407-X,
author = {Bonnin, RA and Creton, E and Perrin, A and Girlich, D and Emeraud, C and Jousset, AB and Duque, M and Jacquemin, A and Hopkins, K and Bogaerts, P and Glupczynski, Y and Pfennigwerth, N and Gniadkowski, M and Hendrickx, APA and van, der Zwaluw K and Apfalter, P and Hartl, R and Studentova, V and Hrabak, J and Larrouy-Maumus, G and Rocha, EPC and Naas, T and Dortet, L},
doi = {10.1016/S2666-5247(23)00407-X},
journal = {Lancet Microbe},
pages = {e547--e558},
title = {Spread of carbapenemase-producing Morganella spp from 2013 to 2021: a comparative genomic study.},
url = {http://dx.doi.org/10.1016/S2666-5247(23)00407-X},
volume = {5},
year = {2024}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - BACKGROUND: Morganella spp are opportunistic pathogens involved in various infections. Intrinsic resistance to multiple antibiotics (including colistin) combined with the emergence of carbapenemase producers reduces the number of active antimicrobials. The aim of this study was to characterise genetic features related to the spread of carbapenem-resistant Morganella spp. METHODS: This comparative genomic study included extensively drug-resistant Morganella spp isolates collected between Jan 1, 2013, and March 1, 2021, by the French National Reference Center (NRC; n=68) and European antimicrobial resistance reference centres in seven European countries (n=104), as well as one isolate from Canada, two reference strains from the Pasteur Institute collection (Paris, France), and two colistin-susceptible isolates from Bicêtre Hospital (Kremlin-Bicêtre, France). The isolates were characterised by whole-genome sequencing, antimicrobial susceptibility testing, and biochemical tests. Complete genomes from GenBank (n=103) were also included for genomic analysis, including phylogeny and determination of core genomes and resistomes. Genetic distance between different species or subspecies was performed using average nucleotide identity (ANI). Intrinsic resistance mechanisms to polymyxins were investigated by combining genetic analysis with mass spectrometry on lipid A. FINDINGS: Distance analysis by ANI of 275 isolates identified three groups: Morganella psychrotolerans, Morganella morganii subspecies sibonii, and M morganii subspecies morganii, and a core genome maximum likelihood phylogenetic tree showed that the M morganii isolates can be separated into four subpopulations. On the basis of these findings and of phenotypic divergences between isolates, we propose a modified taxonomy for the Morganella genus including four species, Morganella psychrotolerans, Morganella sibonii, Morganella morganii, and a new species represented by a unique environmental isolate. W
AU - Bonnin,RA
AU - Creton,E
AU - Perrin,A
AU - Girlich,D
AU - Emeraud,C
AU - Jousset,AB
AU - Duque,M
AU - Jacquemin,A
AU - Hopkins,K
AU - Bogaerts,P
AU - Glupczynski,Y
AU - Pfennigwerth,N
AU - Gniadkowski,M
AU - Hendrickx,APA
AU - van,der Zwaluw K
AU - Apfalter,P
AU - Hartl,R
AU - Studentova,V
AU - Hrabak,J
AU - Larrouy-Maumus,G
AU - Rocha,EPC
AU - Naas,T
AU - Dortet,L
DO - 10.1016/S2666-5247(23)00407-X
EP - 558
PY - 2024///
SP - 547
TI - Spread of carbapenemase-producing Morganella spp from 2013 to 2021: a comparative genomic study.
T2 - Lancet Microbe
UR - http://dx.doi.org/10.1016/S2666-5247(23)00407-X
UR - https://www.ncbi.nlm.nih.gov/pubmed/38677305
VL - 5
ER -