In this section
@article{Tica:2024:10.1021/acssynbio.4c00068,
author = {Tica, J and Chen, H and LUO, S and Chen, M and Isalan, M},
doi = {10.1021/acssynbio.4c00068},
journal = {ACS Synthetic Biology},
pages = {1750--1761},
title = {Engineering tunable, low latency spatial computation with dual input quorum sensing promoters},
url = {http://dx.doi.org/10.1021/acssynbio.4c00068},
volume = {13},
year = {2024}
}
TY - JOUR
AB - Quorum sensing signals have evolved for population-level signaling in bacterial communities and are versatile tools for engineering cell–cell signaling in synthetic biology projects. Here, we characterize the spatial diffusion of a palette of quorum sensing signals and find that their diffusion in agar can be predicted from their molecular weight with a simple power law. We also engineer novel dual- and multi-input promoters that respond to quorum-sensing diffusive signals for use in engineered genetic systems. We engineer a promoter scaffold that can be adapted for activation and repression by multiple diffusers simultaneously. Lastly, we combine the knowledge on diffusion dynamics with the novel genetic components to build a new generation of spatial, stripe-forming systems with a simplified design, improved robustness, tuneability, and response time.
AU - Tica,J
AU - Chen,H
AU - LUO,S
AU - Chen,M
AU - Isalan,M
DO - 10.1021/acssynbio.4c00068
EP - 1761
PY - 2024///
SN - 2161-5063
SP - 1750
TI - Engineering tunable, low latency spatial computation with dual input quorum sensing promoters
T2 - ACS Synthetic Biology
UR - http://dx.doi.org/10.1021/acssynbio.4c00068
UR - https://pubs.acs.org/doi/10.1021/acssynbio.4c00068
UR - http://hdl.handle.net/10044/1/111885
VL - 13
ER -
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