BibTex format
@article{Kourelis:2024:plcell/koae183,
author = {Kourelis, J and Schuster, M and Demir, F and Mattinson, O and Krauter, S and Kahlon, PS and O'Grady, R and Royston, S and Bravo-Cazar, AL and Mooney, BC and Huesgen, PF and Kamoun, S and van, der Hoorn RAL},
doi = {plcell/koae183},
journal = {Plant Cell},
pages = {3260--3276},
title = {Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors.},
url = {http://dx.doi.org/10.1093/plcell/koae183},
volume = {36},
year = {2024}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Secreted immune proteases "Required for Cladosporium resistance-3" (Rcr3) and "Phytophthora-inhibited protease-1" (Pip1) of tomato (Solanum lycopersicum) are both inhibited by Avirulence-2 (Avr2) from the fungal plant pathogen Cladosporium fulvum. However, only Rcr3 acts as a decoy co-receptor that detects Avr2 in the presence of the Cf-2 immune receptor. Here, we identified crucial residues in tomato Rcr3 that are required for Cf-2-mediated signaling and bioengineered various proteases to trigger Avr2/Cf-2-dependent immunity. Despite substantial divergence in Rcr3 orthologs from eggplant (Solanum melongena) and tobacco (Nicotiana spp.), minimal alterations were sufficient to trigger Avr2/Cf-2-mediated immune signaling. By contrast, tomato Pip1 was bioengineered with 16 Rcr3-specific residues to initiate Avr2/Cf-2-triggered immune signaling. These residues cluster on one side of the protein next to the substrate-binding groove, indicating a potential Cf-2 interaction site. Our findings also revealed that Rcr3 and Pip1 have distinct substrate preferences determined by two variant residues and that both are suboptimal for binding Avr2. This study advances our understanding of Avr2 perception and opens avenues to bioengineer proteases to broaden pathogen recognition in other crops.
AU - Kourelis,J
AU - Schuster,M
AU - Demir,F
AU - Mattinson,O
AU - Krauter,S
AU - Kahlon,PS
AU - O'Grady,R
AU - Royston,S
AU - Bravo-Cazar,AL
AU - Mooney,BC
AU - Huesgen,PF
AU - Kamoun,S
AU - van,der Hoorn RAL
DO - plcell/koae183
EP - 3276
PY - 2024///
SP - 3260
TI - Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors.
T2 - Plant Cell
UR - http://dx.doi.org/10.1093/plcell/koae183
UR - https://www.ncbi.nlm.nih.gov/pubmed/38923940
VL - 36
ER -