In this section
@article{Boussac:1988:10.1016/0014-5793(88)80071-1,
author = {Boussac, A and Rutherford, AW},
doi = {10.1016/0014-5793(88)80071-1},
journal = {FEBS Letters},
pages = {432--436},
title = {Ca2+ binding to the oxygen evolving enzyme varies with the redox state of the Mn cluster},
url = {http://dx.doi.org/10.1016/0014-5793(88)80071-1},
volume = {236},
year = {1988}
}
TY - JOUR
AB - Oxygen evolution by the mangano-enzyme of photosystem II is inhibited by Ca<sup>2+</sup> depletion induced by NaCl washing and restored by Ca<sup>2+</sup> addition. The effectiveness of NaCl treatment in inhibiting oxygen evolution in photosystem II was studied after a series of preilluminating flashes. The susceptibility of the enzyme to NaCl treatment varied with the number of preilluminating flashes and this variation showed an oscillation pattern with a period of four. This pattern is characteristic of cycling through the four long-lived intermediate states in the enzyme cycle (i.e. the states, S<inf>0</inf>, S<inf>1</inf>, S<inf>2</inf>, S<inf>3</inf>). The relative extent of inhibition corresponding to each of the S states was as follows: S<inf>3</inf> > S<inf>0</inf>≈S<inf>2</inf> > S<inf>1</inf>. From these results it is concluded that Ca<sup>2+</sup> binding is dependent on the S states and that Ca<sup>2+</sup> probably plays a fundamental role in the mechanism of water splitting. The results also help to explain the conflicting reports of the extent of inhibition induced by NaCl washing and the controversy over which electron transfer step is inhibited by Ca<sup>2+</sup> depletion. © 1988.
AU - Boussac,A
AU - Rutherford,AW
DO - 10.1016/0014-5793(88)80071-1
EP - 436
PY - 1988///
SN - 0014-5793
SP - 432
TI - Ca2+ binding to the oxygen evolving enzyme varies with the redox state of the Mn cluster
T2 - FEBS Letters
UR - http://dx.doi.org/10.1016/0014-5793(88)80071-1
VL - 236
ER -
Interested in studying a PhD at the Department of Life Sciences? Find out more about postgraduate research opportunties.