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@article{Ono:1986:10.1016/0005-2728(86)90125-8,
author = {Ono, T and Zimmermann, JL and Inoue, Y and Rutherford, AW},
doi = {10.1016/0005-2728(86)90125-8},
journal = {Bba Bioenergetics},
pages = {193--201},
title = {EPR evidence for a modified S-state transition in chloride-depleted Photosystem II},
url = {http://dx.doi.org/10.1016/0005-2728(86)90125-8},
volume = {851},
year = {1986}
}
TY - JOUR
AB - The role of chloride on the S-state transition in spinach Photosystem II (PS II) particles was investigated by EPR spectroscopy at low temperature and the following results were obtained. (1) After excitation by continuous light at 200 K, chloride-depleted particles did not show the EPR multiline signal associated with the S<inf>2</inf> state, but only showed the broad signal at g = 4.1. The S<inf>2</inf> multiline signal was completely restored upon chloride repletion. (2) In the absence of chloride the S<inf>2</inf> multiline signal was not induced by a single flash excitation at 0°C. However, upon addition of chloride after the flash the signal was developed in darkness. (3) The amplitude of the multiline S<inf>2</inf> signal thus developed upon chloride addition after flash illumination did not show oscillations dependent upon flash number. These results indicate that the O<inf>2</inf>-evolving complex in chloride-depleted PS II membranes is able to store at least one oxidizing equivalent, a modified S<inf>2</inf> state, which does not give rise to the multiline signal. Addition of chloride converts this oxidizing equivalent to the normal S<inf>2</inf> state which gives rise to the multiline signal. The modified S<inf>2</inf> state is more stable than the normal S<inf>2</inf> state, showing decay kinetics about 20-times slower than those of the normal S<inf>2</inf> state, and the formation of higher S states is blocked. © 1986.
AU - Ono,T
AU - Zimmermann,JL
AU - Inoue,Y
AU - Rutherford,AW
DO - 10.1016/0005-2728(86)90125-8
EP - 201
PY - 1986///
SN - 0005-2728
SP - 193
TI - EPR evidence for a modified S-state transition in chloride-depleted Photosystem II
T2 - Bba Bioenergetics
UR - http://dx.doi.org/10.1016/0005-2728(86)90125-8
VL - 851
ER -
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