In this section
@article{Walter:2024:10.1039/d3sc06445j,
author = {Walter, ERH and Lee, LC-C and Leung, PK-K and Lo, KK-W and Long, NJ},
doi = {10.1039/d3sc06445j},
journal = {Chemical Science},
pages = {4846--4852},
title = {Mitochondria-targeting biocompatible fluorescent BODIPY probes},
url = {http://dx.doi.org/10.1039/d3sc06445j},
volume = {15},
year = {2024}
}
TY - JOUR
AB - An increase in the mitochondrial membrane potential (MMP) is a characteristic feature of cancer and cardiovascular disease. Therefore, it remains of crucial importance to develop new and improved fluorescent probes that are sensitive to the MMP, to report on mitochondrial health and function. Reported here are the design, synthesis, photophysical properties and biological characterisation of a series of BODIPY dyes, BODIPY-Mito-n, for mitochondria-targeted fluorescence imaging applications. Six BODIPY-Mito-n analogues were synthesised under mild conditions, and displayed excellent fluorescence quantum yields of between 0.59 and 0.72 in aqueous environments at physiological pH (pH = 7.4). The incorporation of poly(ethylene glycol) (PEG) chains to the triarylphosphonium cation moiety significantly improved the biocompatibility of the probes (BODIPY-Mito-6, IC50 > 50 μM). All BODIPY-Mito-n compounds demonstrated a high MMP-sensitive localisation in the mitochondria, with Pearson's correlation coefficients (PCC) of between 0.76 and 0.96. Compounds BODIPY-Mito-2 and BODIPY-Mito-6 revealed the highest sensitivity to the MMP, with a decrease in the emission intensity of 62% and 75%, respectively following MMP depolarisation. It is anticipated that the highest MMP sensitivity and enhanced biocompatibility of BODIPY-Mito-6 could lead to the development of new probes for mitochondrial imaging in the future.
AU - Walter,ERH
AU - Lee,LC-C
AU - Leung,PK-K
AU - Lo,KK-W
AU - Long,NJ
DO - 10.1039/d3sc06445j
EP - 4852
PY - 2024///
SN - 2041-6520
SP - 4846
TI - Mitochondria-targeting biocompatible fluorescent BODIPY probes
T2 - Chemical Science
UR - http://dx.doi.org/10.1039/d3sc06445j
UR - https://www.ncbi.nlm.nih.gov/pubmed/38550684
UR - https://pubs.rsc.org/en/content/articlelanding/2024/sc/d3sc06445j
UR - http://hdl.handle.net/10044/1/112153
VL - 15
ER -
Professor Nick Long
Email: n.long@imperial.ac.uk
Telephone: +44 (0)20 7594 5781
501J
Molecular Sciences Research Hub
White City Campus
