In this section
@article{Du:2017:10.1039/c7dt02418e,
author = {Du, S and Hernandez-Gil, J and Dong, H and Zheng, X and Lyu, G and Banobre-Lopez, M and Gallo, J and Sun, L-D and Yan, C-H and Long, NJ},
doi = {10.1039/c7dt02418e},
journal = {Dalton Transactions},
pages = {13957--13965},
title = {Design and validation of a new ratiometric intracellular pH imaging probe using lanthanide-doped upconverting nanoparticles},
url = {http://dx.doi.org/10.1039/c7dt02418e},
volume = {46},
year = {2017}
}
TY - JOUR
AB - pH homeostasis is strictly controlled at a subcellular level. A deregulation of the intra/extra/subcellular pH environment is associated with a number of diseases and as such, the monitoring of the pH state of cells and tissues is a valuable diagnostic tool. To date, only a few tools have been developed to measure the pH in living cells with the spatial resolution needed for intracellular imaging. Among the techniques available, only optical imaging offers enough resolution and biocompatibility to be proposed for subcellular pH monitoring. We present herein a ratiometric probe based on upconversion nanoparticles modified with a pH sensitive moiety for the quantitative imaging of pH at the subcellular level in living cells. This system provides the properties required for live cell quantitative imaging i.e. positive cellular uptake, biocompatibility, long wavelength excitation, sensitive response to pH within a biologically relevant range, and self-referenced signal.
AU - Du,S
AU - Hernandez-Gil,J
AU - Dong,H
AU - Zheng,X
AU - Lyu,G
AU - Banobre-Lopez,M
AU - Gallo,J
AU - Sun,L-D
AU - Yan,C-H
AU - Long,NJ
DO - 10.1039/c7dt02418e
EP - 13965
PY - 2017///
SN - 1477-9234
SP - 13957
TI - Design and validation of a new ratiometric intracellular pH imaging probe using lanthanide-doped upconverting nanoparticles
T2 - Dalton Transactions
UR - http://dx.doi.org/10.1039/c7dt02418e
UR - http://hdl.handle.net/10044/1/55248
VL - 46
ER -
Professor Nick Long
Email: n.long@imperial.ac.uk
Telephone: +44 (0)20 7594 5781
501J
Molecular Sciences Research Hub
White City Campus
