BibTex format
@article{Martinez-Sanchez:2018:10.1096/fj.201701100R,
author = {Martinez-Sanchez, A and Nguyen-Tu, M-S and Cebola, I and Yavari, A and Marchetti, P and Piemonti, L and de, Koning E and Shapiro, AMJ and Johnson, P and Sakamoto, K and Smith, DM and Leclerc, I and Ashrafian, H and Ferrer, J and Rutter, GA},
doi = {10.1096/fj.201701100R},
journal = {FASEB Journal},
pages = {2587--2600},
title = {MiR-184 expression is regulated by AMPK in pancreatic islets.},
url = {http://dx.doi.org/10.1096/fj.201701100R},
volume = {32},
year = {2018}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - AMPK is a critical energy sensor and target for widely used antidiabetic drugs. In β-cells, elevated glucose concentrations lower AMPK activity, and the ablation of both catalytic subunits (βAMPKdKO mice) impairs insulin secretion in vivo and β-cell identity. MicroRNAs (miRNAs) are small RNAs that silence gene expression that are essential for pancreatic β-cell function and identity and altered in diabetes. Here, we have explored the miRNAs acting downstream of AMPK in mouse and human β-cells. We identified 14 down-regulated and 9 up-regulated miRNAs in βAMPKdKO vs. control islets. Gene ontology analysis of targeted transcripts revealed enrichment in pathways important for β-cell function and identity. The most down-regulated miRNA was miR-184 (miR-184-3p), an important regulator of β-cell function and compensatory expansion that is controlled by glucose and reduced in diabetes. We demonstrate that AMPK is a potent regulator and an important mediator of the negative effects of glucose on miR-184 expression. Additionally, we reveal sexual dimorphism in miR-184 expression in mouse and human islets. Collectively, these data demonstrate that glucose-mediated changes in AMPK activity are central for the regulation of miR-184 and other miRNAs in islets and provide a link between energy status and gene expression in β-cells.-Martinez-Sanchez, A., Nguyen-Tu, M.-S., Cebola, I., Yavari, A., Marchetti, P., Piemonti, L., de Koning, E., Shapiro, A. M. J., Johnson, P., Sakamoto, K., Smith, D. M., Leclerc, I., Ashrafian, H., Ferrer, J., Rutter, G. A. MiR-184 expression is regulated by AMPK in pancreatic islets.
AU - Martinez-Sanchez,A
AU - Nguyen-Tu,M-S
AU - Cebola,I
AU - Yavari,A
AU - Marchetti,P
AU - Piemonti,L
AU - de,Koning E
AU - Shapiro,AMJ
AU - Johnson,P
AU - Sakamoto,K
AU - Smith,DM
AU - Leclerc,I
AU - Ashrafian,H
AU - Ferrer,J
AU - Rutter,GA
DO - 10.1096/fj.201701100R
EP - 2600
PY - 2018///
SN - 0892-6638
SP - 2587
TI - MiR-184 expression is regulated by AMPK in pancreatic islets.
T2 - FASEB Journal
UR - http://dx.doi.org/10.1096/fj.201701100R
UR - http://hdl.handle.net/10044/1/63379
VL - 32
ER -
