HCF3 - Leica Stellaris STED FALCON

 Leica STELLARIS Tau-STED FALCON FLIM/ FCCS/ FLCS

Advantages of this microscope

• Tau-STED 2D/3D + 3 Depletion Lasers - 592, 660, 775nm
• STED White Objectives - 100x 1.40NA Oil + 86x 1.20NA Water (Motorised Correction)
• FALCON FLIM + FCCS + FLCS
• Phasor Analysis
• Confocal Super Resolution LASX Lightning Expert
• DMi8 CS Inverted Motorised Microscope
• Incubation Chamber - Cage Type
• Stage Top Incubator (Z-Galvanometer), CO2 Mixer and Humidity
• BF Detector for DMi 8
• LED Epiflfluorescence
• XY Scanning Stage + Z Galvanometer Stage - LASX Navigator Software
• White Laser 440-790nm + Pulse Picker
• 405nm Diode
• 8 kHz Tandem Scanner + FOV Scanner - Software Switching
• 4 Channel Spectral Dectection - 2 HyDS + HyDX + HyDR

Hammersmith Campus, ICTEM (L-Block) room 312
SN:8300000358

NB All users must have completed the on-line “Introduction to Laser Safety” from Imperial College safety department

Quickstart guides

Quickstart guide - Confocal HCF3 Leica Stellaris 8 Inverted (PDF)‌

Quickstart guide - STED HCF3 Leica Stellaris 8 Inverted (PDF)‌

Tiling and Multi-position Imaging (PDF)

STED

Leica Tutorial - Tau-STED.  Explains the theory and also how to perform the Tau-STED experiments within the software: 

 STED Sample Preparation (PDF)‌‌

FALCON

Phasor-FLIM (YouTube)  Phasor-FLIM (highly recommend, a very good seminar from the original group (Enrico Gratton)  FRET trajectory is also explained.

Leica Tutorials - FALCON Phasor analysis.  In the tutorial, many aspects of the software are covered and a tutorial on how to calculate FRET using Phasors on Alexa488 Tubulin and Alexa 555 Tubulin example data and removing unwanted signals using the phasor approach

Application Note:  SP8 FALCON: a novel concept in fluorescence lifetime imaging enabling video-rate confocal FLIM

‌

‌Lightning White Paper (PDF)

‌

‌

 

Tau Sense (PDF)

Leica Objectives‌‌‌‌

Offline software

• Leica LAS X Lite
• FIJI