@article{Rodriguez-Manzano:2019:10.1021/acs.analchem.8b04412,
author = {Rodriguez-Manzano, J and Moniri, A and Malpartida-Cardenas, K and Dronavalli, J and Davies, F and Holmes, A and Georgiou, P},
doi = {10.1021/acs.analchem.8b04412},
journal = {Analytical Chemistry},
pages = {2013--2020},
title = {Simultaneous single-channel multiplexing and quantification of carbapenem-resistant genes using multidimensional standard curves},
url = {http://dx.doi.org/10.1021/acs.analchem.8b04412},
volume = {91},
year = {2019}
}

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TY  - JOUR
AB  - Multiplexing and quantification of nucleic acids, both have, in their own right, significant and extensive use in biomedical related fields. Currently, the ability to detect several nucleic acid targets in a single-reaction scales linearly with the number of targets; an expensive and time-consuming feat. Here, we propose a new methodology based on multidimensional standard curves that extends the use of real-time PCR data obtained by common qPCR instruments. By applying this novel method-ology, we achieve simultaneous single-channel multiplexing and enhanced quantification of multiple targets using only real-time amplification data. This is obtained without the need of fluorescent probes, agarose gels, melting curves or sequencing analysis. Given the importance and demand for tackling challenges in antimicrobial resistance, the proposed method is ap-plied to four of the most prominent carbapenem-resistant genes: blaOXA-48, blaNDM, blaVIM and blaKPC, which account for 97% of the UK's reported carbapenemase-producing Enterobacteriaceae.
AU  - Rodriguez-Manzano,J
AU  - Moniri,A
AU  - Malpartida-Cardenas,K
AU  - Dronavalli,J
AU  - Davies,F
AU  - Holmes,A
AU  - Georgiou,P
DO  - 10.1021/acs.analchem.8b04412
EP  - 2020
PY  - 2019///
SN  - 0003-2700
SP  - 2013
TI  - Simultaneous single-channel multiplexing and quantification of carbapenem-resistant genes using multidimensional standard curves
T2  - Analytical Chemistry
UR  - http://dx.doi.org/10.1021/acs.analchem.8b04412
UR  - https://www.ncbi.nlm.nih.gov/pubmed/30624047
UR  - http://hdl.handle.net/10044/1/65997
VL  - 91
ER  - 

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