BibTex format
@article{Boonyasiri:2021:10.1186/s12879-021-05790-9,
author = {Boonyasiri, A and Jauneikaite, E and Brinkac, LM and Greco, C and Lerdlamyong, K and Tangkoskul, T and Nguyen, K and Thamlikitkul, V and Fouts, DE},
doi = {10.1186/s12879-021-05790-9},
journal = {BMC Infectious Diseases},
pages = {1--11},
title = {Genomic and clinical characterisation of multidrug-resistant carbapenemase-producing ST231 and ST16 Klebsiella pneumoniae isolates colonising patients at Siriraj hospital, Bangkok, Thailand from 2015 to 2017.},
url = {http://dx.doi.org/10.1186/s12879-021-05790-9},
volume = {21},
year = {2021}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - BACKGROUND: Infections caused by carbapenemase-producing Enterobacteriaceae (CPE) have continually grown as a global public health threat, with significant mortality rates observed across the world. We examined the clinical data from patients with CPE infections and their outcomes, concentrating on Klebsiella pneumoniae isolates. We analysed the clinical information, performed antimicrobial susceptibility testing, and conducted molecular epidemiological and genomic analyses on the isolates to identify patterns in the data. METHODS: The clinical characteristics of 33 hospitalised patients with confirmed CPE, including patient-related factors associated with the development of CPE infections, were examined. Patients were divided according to whether they were "colonised" or "infected" with CPE and by the timing and frequency of their rectal swab collections, from which 45 swabs were randomly selected for analysis. CPE isolates were purified, and antimicrobial susceptibility tests performed. Whole genome sequences of these isolates were determined and analysed to compute bacterial multilocus sequence types and plasmid replicon types, infer phylogenetic relationships, and identify antimicrobial resistance and virulence genes. RESULTS: Altogether, 88.9% (40/45) of the CPE isolates were K. pneumoniae. The most abundant carbapenemase gene family in the K. pneumoniae isolates (33/39) was blaOXA-232, with blaNDM-1 additionally identified in 19 of them. All CPE isolates carrying either blaOXA-232 or blaNDM-1 were resistant to meropenem, but only 40 from 45 were susceptible to colistin. Among the CPE-infected patients (n = 18) and CPE-colonised patients who developed CPE infections during the study (n = 3), all but one received standard colistin-based combination therapy. Phylogenetic analysis revealed the polyclonal spread of carbapenemase-producing K. pneumoniae (CPKP) within the patient population, with the following two major
AU - Boonyasiri,A
AU - Jauneikaite,E
AU - Brinkac,LM
AU - Greco,C
AU - Lerdlamyong,K
AU - Tangkoskul,T
AU - Nguyen,K
AU - Thamlikitkul,V
AU - Fouts,DE
DO - 10.1186/s12879-021-05790-9
EP - 11
PY - 2021///
SN - 1471-2334
SP - 1
TI - Genomic and clinical characterisation of multidrug-resistant carbapenemase-producing ST231 and ST16 Klebsiella pneumoniae isolates colonising patients at Siriraj hospital, Bangkok, Thailand from 2015 to 2017.
T2 - BMC Infectious Diseases
UR - http://dx.doi.org/10.1186/s12879-021-05790-9
UR - https://www.ncbi.nlm.nih.gov/pubmed/33541274
UR - https://bmcinfectdis.biomedcentral.com/articles/10.1186/s12879-021-05790-9
UR - http://hdl.handle.net/10044/1/86913
VL - 21
ER -
