BibTex format
@article{Ledda:2022:10.1099/mgen.0.000675,
author = {Ledda, A and Cummins, M and Shaw, LP and Jauneikaite, E and Cole, K and Lasalle, F and Barry, D and Turton, J and Rosmarin, C and Anaraki, S and Wareham, D and Stoesser, N and Paul, J and Manuel, R and Cherian, BP and Didelot, X},
doi = {10.1099/mgen.0.000675},
journal = {Microbial Genomics},
title = {Hospital outbreak of carbapenem-resistant Enterobacterales associated with a bla OXA-48 plasmid carried mostly by Escherichia coli ST399},
url = {http://dx.doi.org/10.1099/mgen.0.000675},
volume = {8},
year = {2022}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - A hospital outbreak of carbapenem-resistant Enterobacterales was detected by routine surveillance. Whole genome sequencing and subsequent analysis revealed a conserved promiscuous blaOXA-48 carrying plasmid as the defining factor within this outbreak. Four different species of Enterobacterales were involved in the outbreak. Escherichia coli ST399 accounted for 35 of all the 55 isolates. Comparative genomics analysis using publicly available E. coli ST399 genomes showed that the outbreak E. coli ST399 isolates formed a unique clade. We developed a mathematical model of pOXA-48-like plasmid transmission between host lineages and used it to estimate its conjugation rate, giving a lower bound of 0.23 conjugation events per lineage per year. Our analysis suggests that co-evolution between the pOXA-48-like plasmid and E. coli ST399 could have played a role in the outbreak. This is the first study to report carbapenem-resistant E. coli ST399 carrying blaOXA-48 as the main cause of a plasmid-borne outbreak within a hospital setting. Our findings suggest complementary roles for both plasmid conjugation and clonal expansion in the emergence of this outbreak.
AU - Ledda,A
AU - Cummins,M
AU - Shaw,LP
AU - Jauneikaite,E
AU - Cole,K
AU - Lasalle,F
AU - Barry,D
AU - Turton,J
AU - Rosmarin,C
AU - Anaraki,S
AU - Wareham,D
AU - Stoesser,N
AU - Paul,J
AU - Manuel,R
AU - Cherian,BP
AU - Didelot,X
DO - 10.1099/mgen.0.000675
PY - 2022///
SN - 2057-5858
TI - Hospital outbreak of carbapenem-resistant Enterobacterales associated with a bla OXA-48 plasmid carried mostly by Escherichia coli ST399
T2 - Microbial Genomics
UR - http://dx.doi.org/10.1099/mgen.0.000675
UR - http://hdl.handle.net/10044/1/96347
VL - 8
ER -
