A Leica SP5 upright confocal microscope with a tunable infrared laser for multiphoton and lifetime imaging (Spectraphysics Mai Tai 690-1020), Flim detector (Becker Hickl) and a motorised stage.
Advantages of this microscope
- multiphoton laser for deep tissue penetration, maximum confocality.
- 4 other lasers (Argon 488, Diode 543, Diode 594, Diode 633)
- Flim detector for fluorescence lifetime imaging (Flim/FRET, intracellular environment)
- Automatic multipoint 4D imaging
- 2 sensitive HyD detectors for increased sensitivity and photon-counting experiments.
- Quad-non-descanned detectors (NDD) for 4-color simultaneous imaging using multiphoton
Location
Sir Alexander Fleming building, SAF 408
SN: 5100000307
Quickstart guide
Quickstart guide - Confocal 3 Leica SP5 Upright (PDF)
Quickstart guide - Multiphoton Laser (PDF)
Hardware configuration
Please see the FILM wiki page - CF3 hardware configuration
Offline software
Booking guidelines, Charges, make a booking
General enquiries
FILM
Sir Alexander Fleming Building
South Kensington Campus
Imperial College London
Exhibition Road
London SW7 2AZ, UK
film-service@imperial.ac.uk