Aria sorter in hood

 

 

405nm Violet

Collection filterMirrorFluorochromes
450/50 - Alexa Fluor 405, BD Horizon V450, BV 421, Calcein Violet, Calcein Violet 450AM, Celltrace Violet, CFP, DAPI, DyLight 405, DyeCycle Violet, eFluor 450, Fixable Violet Dead Cell Stain, Fixable Viability eFluor 450, FxCycle Violet, Hoechst (blue), Pacific Blue, PO-PRO-1, SYTOX Blue, VioBlue
525/50 475LP AmCyan, BD Horizon V500, Brilliant Violet 510, Fixable Aqua Dead cell stain, Fixable Viability eFluor 506, Pacific Orange, Qdot 525, VioGreen
605/12 595LP BV605
660/40 630LP BV650
720/20 700LP BV711
780/60 735LP BV786, Qdot 800
Summary of the table's contents

488nm Blue

Collection filterMirrorFluorochromes
525/50 505LP AlexaFluor 488, Calcein, CellVue Jade, CFSE, Cy2, DyLight 488, DyeCycle Green, eGFP,  eYFP , FITC, Fixable green dead cell stain, SYTOX Green, TO-PRO-1, YO-PRO-1
710/50 685LP 7-AAD,  PerCP-Cy5.5, PerCP-eFluor710
Summary of the table's contents

561nm Yellow Green

Collection filterMirrorFluorochromes
582/15 570LP PE, DsRed, tdTomato, RFP
620/10 600LP  m-Cherry, PE-Texas Red, PI, PE-CF594, Texas Red
710/50 685LP 7-AAD, Pe-Cy5.5,  PI
780/60 755LP PE-Cy7, PE-Vio 770
Summary of the table's contents

633nm Red

Collection filterMirrorFluorochromes
670/30 - APC, Alexa Fluor 647, Cell Proliferation Dye eFluor 670, Cy5.5, DRAQ5, DRAQ7, Dylight 649, Fixable far red dead stain, Fixable Viability eFluor 660, Fx Cycle Far Red Stain, Oyster 650, SYTOX Red, TO-PRO-3, YO-PRO-3
730/45 685LP Alexa Fluor 700, APC-Cy5.5, DRAQ7, Dye Cycle Ruby
780/60 755LP Alexa Fluor 750, APC-Alexa Fluor 750, APC-Cy7, APC-eFluor 780, APC-H7, APC-Vio770, CellVue NIR780, DRAQ7, Fixable near-IR dead stain,, Fixable Viability eFluor 780
Note: Not every fluorochrome will be able to be used in combination with the ones above, it is the responsibility of the user to research this during panel setup.
National Heart and Lung Institute

General enquiries


St Mary's FACS Facility
Room 312D, third floor
Medical school building 
St Mary's Campus 
Norfolk Place 
Paddington 
London W2 1PG 

Facility Manager
Dr Isabel Correa-Otero
i.correa-otero@imperial.ac.uk