355nm UV
Collection filter | Mirror | Fluorochromes |
---|---|---|
450/50* | - | DAPI, Calcein Blue AM, Fixable Blue Dead Cell Stain, Indo-1 hi, Indo-1 AM, Hoechst 33258, NucBlue Live Cell Stain |
530/30** | 505LP | Alexa Fluor 430, Indo-1 lo, Qdot525 |
*450/50 filter can be changed for 379/28 filter for BUV395; **530/30 filter can be changed for 740/35 and LP690 for BUV737 |
405nm Violet
Collection filter | Mirror | Fluorochromes |
---|---|---|
450/50 | - | BV421, Alexa Fluor 405, BD Horizon V450, Celltrace Violet, CFP, DAPI, DyLight 405, DyeCycle Violet, eFluor 450, Fixable Violet Dead Cell Stain, Fixable Viability eFluor 450, Hoechst (blue), Pacific Blue, PO-PRO-1, SYTOX Blue, VioBlue |
525/50 | 475LP | BV510, AmCyan, BD Horizon V500, Fixable Aqua Dead cell stain, Fixable Viability eFluor 506, Krome Orange, Pacific Orange, Qdot 525, VioGreen |
605/12 | 595LP | BV605, Qdot 585 |
655/8 | 630LP | BV650 |
710/50 | 690LP | Brilliant Violet 711, Qdot 705 |
780/60 | 750LP | Brilliant Violet 786, Qdot 800 |
488nm Blue
Collection filter | Mirror | Fluorochromes |
---|---|---|
530/30 | 505LP | FITC, AlexaFluor 488, Calcein, CellVue Jade, CFSE, Cy2, DyLight 488, DyeCycle Green, eGFP, eYFP , Fixable green dead cell stain, mCitrine, Oyster 488, Rhodamine 110, Rhodamine 123, SYTOX Green, TO-PRO-1, YO-PRO-1 |
675/20 | 660LP | PerCP, (PE-Cy5**) |
710/50 | 685LP | 7-AAD, PerCP-Cy5.5, PerCP-eFluor710 |
**PE-Cy5 is not recommended for use with APC. |
561nm Yellow Green
Collection filter | Mirror | Fluorochromes |
---|---|---|
582/15 | 570LP | PE, DsRed, tdTomato, RFP |
620/10 | 600LP | PE-Dazzle 594, PE-Texas Red, m-Cherry, PI, PE-Vio615 |
710/50 | 685LP | 7-AAD, PE-Cy5.5, |
780/60 | 750LP | PE-Cy7, PE-Vio 770 |
Note: Not every fluorochrome will be able to be used in combination with the ones above, it is the responsibility of the user to research this during panel setup. |
633nm Red
Collection filter | Mirror | Fluorochromes |
---|---|---|
670/14 | - | APC, Alexa Fluor 647, Cell Proliferation Dye eFluor 670, CellVue Maroon, CellVue Plum, Cy5, Cy5.5, DRAQ5, DRAQ7, Dylight 649, Dye Cycle Ruby, Fixable far red dead stain, Fixable Viability eFluor 660, Fx Cycle Far Red Stain, Oyster 650, SYTOX Red, TO-PRO-3, YO-PRO-3 |
720/40 | 710LP | Alexa Fluor 700, APC-Cy5.5, CellVue Burgundy, DRAQ7, Dye Cycle Ruby |
780/60 | 750LP | Alexa Fluor 750, APC-Alexa Fluor 750, APC-Cy7, APC-eFluor 780, APC-H7, APC-Vio770, CellVue NIR780, DRAQ7, Fixable near-IR dead stain,, Fixable Viability eFluor 780 |
Note: Not every fluorochrome will be able to be used in combination with the ones above, it is the responsibility of the user to research this during panel setup. |
Useful links
General enquiries
St Mary's FACS Facility
Room 312D, third floor
Medical school building
St Mary's Campus
Norfolk Place
Paddington
London W2 1PG
Facility Manager
Dr Isabel Correa-Otero
i.correa-otero@imperial.ac.uk