@article{Bernardo:2012:10.1016/j.humimm.2011.12.017,
author = {Bernardo, D and Al-Hassi, HO and Mann, ER and Tee, CT and Murugananthan, AU and Peake, STC and Hart, AL and Knight, SC},
doi = {10.1016/j.humimm.2011.12.017},
journal = {Human Imunology},
title = {T-cell proliferation and FoxP3 expression in human T-cells are dependent on T-cell density: physics of a confined space?},
url = {http://dx.doi.org/10.1016/j.humimm.2011.12.017},
year = {2012}
}

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TY  - JOUR
AB  - T-cell proliferation rates in vitro depend on factors including initial T-cell number, dose of stimulus, culture time, and available physical space. The role of forkhead box P3 (FoxP3) in the identification of T cells with a regulatory phenotype remains controversial in humans. Through 5-carboxyfluorescein diacetate succinimidyl ester labeling of human T cells and subsequent culture of different numbers of T cells and antigen-presenting cells (APC), we studied proliferative T-cell responses and FoxP3 expression in divided T cells. T-cell proliferation rates depended on initial T-cell/APC numbers. Proliferation rates decreased when high initial T-cell numbers were increased. FoxP3 expression was expressed exclusively in virtually all divided T cells cultured at high T-cell densities, irrespective of their CD4 nature or cytokine content, and was coexpressed with T-bet. However, when T cells were cultured on larger surfaces or at lower initial numbers, FoxP3 expression was not induced in divided T cells, even when most of the cells had undergone cell division. FoxP3(+) T cells generated at high cell densities did not elicit a suppressive phenotype and FoxP3 expression was subsequently lost in time when the stimulus was removed. Therefore, caution should be observed in the use of FoxP3 expression to identify regulatory T cells in humans because its expression may be only a consequence of activation status in a restricted environment.
AU  - Bernardo,D
AU  - Al-Hassi,HO
AU  - Mann,ER
AU  - Tee,CT
AU  - Murugananthan,AU
AU  - Peake,STC
AU  - Hart,AL
AU  - Knight,SC
DO  - 10.1016/j.humimm.2011.12.017
PY  - 2012///
TI  - T-cell proliferation and FoxP3 expression in human T-cells are dependent on T-cell density: physics of a confined space?
T2  - Human Imunology
UR  - http://dx.doi.org/10.1016/j.humimm.2011.12.017
ER  - 

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