@article{Vora:2016:10.18632/oncotarget.7799,
author = {Vora, R and Bernardo, D and Durant, L and Reddi, D and Hart, AL and Fell, JME and Al-Hassi, HO and Knight, SC},
doi = {10.18632/oncotarget.7799},
journal = {Oncotarget},
pages = {11913--11922},
title = {Age-related alterations in blood and colonic dendritic cell properties},
url = {http://dx.doi.org/10.18632/oncotarget.7799},
volume = {7},
year = {2016}
}
TY - JOUR
AB - Background Dendritic cells (DC) determine initiation, type and location of immuneresponses and, in adults, show decreased Toll-like receptors and some increasedcytokine levels on ageing. Few studies in children have characterised DC orexplored DC-related mechanisms producing age-related immune changes.Methods Blood and colonic DC phenotypes were determined in healthy adults andchildren by flow cytometry and correlated with aging. Blood DC were divided intoplasmacytoid (pDC) and myeloid (mDC) while only mDC were identified in colon.Serum cytokine levels were determined by multiplex cytokine assays and correlatedwith DC properties.Results The pDC marker BDCA2 (but not CD123) was absent in pre-pubertalchildren and numbers of pDC decreased with age. Blood and colonic DC were moremature and activated in adults. Decrease in pDC numbers correlated with reducedGM-CSF levels with aging, but increasing IL-4 and IL-8 levels correlated with a moreactivated DC profile in blood. CXCL16 levels decreased with age.Conclusions In children, lack of BDCA2, a receptor mediating antigen capture andinhibiting interferon induction, may be immunologically beneficial during immunedevelopment. Conversely, reduced pDC numbers, probably secondary todecreasing GM-CSF and increasing cytokine-induced maturation of DC are likely todetermine deteriorating immunity with ageing.
AU - Vora,R
AU - Bernardo,D
AU - Durant,L
AU - Reddi,D
AU - Hart,AL
AU - Fell,JME
AU - Al-Hassi,HO
AU - Knight,SC
DO - 10.18632/oncotarget.7799
EP - 11922
PY - 2016///
SN - 1949-2553
SP - 11913
TI - Age-related alterations in blood and colonic dendritic cell properties
T2 - Oncotarget
UR - http://dx.doi.org/10.18632/oncotarget.7799
UR - http://hdl.handle.net/10044/1/29914
VL - 7
ER -