Agilent NovoCyte Penteon
The Agilent Penteon analyser is equipped with five lasers (UV 349nm, Violet 405nm, Blue 488nm, Yellow-Green 561nm and Red 637nm) and is capable of up to 30 detection channels (30 fluorescence channels, FSC and SSC). It is empowered by Silicon photomultipliers (SiPM), which are silicon-substrate-based semiconductor devices. A wide, 7-log dynamic range eliminates the need for routine detector adjustments
Scatter detection optics and signal processing electronics have been optimised to resolve particles down to 40 nm in size, making it ideal for platelet and bacteria analysis. The system is fully automated to design your experiment, load your samples in the dedicated rack (up to 40 tubes) and walk away while your samples are being run. Plate loading is available for 24-, 48-, 96-, and 384-well formats. It is also capable of performing absolute cell countings.
The data acquisition rate is a maximum of 70,000 events per second. We recommend a minimum sample volume of 300 µL.
Advantages:
- Enhanced detection of smaller particles such as bacteria and platelets due to optimised scatter detection optics and signal processing electronics.
- Increased availability of UV and Violet channels.
- Wide, 7-log dynamic range eliminates the need for routine detector adjustments.
- Accurate enumeration of particles without use of counting beads.
- Walk-away operation to simplify your workday.
For more information, please read the NovoCyte Penteon - User Manual and Agilent website.
Download a complete configuration of Agilent Penteon Lasers and Filters. You can also find it below.
Bandpass filter | Parameter | Fluorochrome |
---|---|---|
445/45 | UV445 | BUV395, Hoescht 33342, DAPI, LD Blue |
525/45 | UV525 | BUV496 |
586/20 | UV586 | BUV563 |
615/20 | UV615 | BUV615 |
667/30 | UV667 | BUV661 |
725/40 | UV725 | BUV737 |
780/60 | UV780 | BUV805 |
Bandpass filter | Parameter | Fluorochrome |
---|---|---|
445/45 | V445 | BV421, DAPI, Pacific Blue, V450, AF405 |
525/45 | V525 | BV510, Zombie/LD Aqua |
586/20 | V586 | BV570, Pacific Orange, LD Yellow |
615/20 | V615 | BV605, Qdot 605 |
667/30 | V667 | BV650, Qdot 655 |
725/40 | V725 | BV711, Qdot 705 |
780/60 | V780 | BV786, Qdot 800 |
Bandpass filter | Parameter | Fluorochrome |
---|---|---|
525/45 | B525 | FITC, AF488, GFP, BB515 |
586/20 | B586 | EYFP, LD Olive |
615/20 | B615 | BB630P, PI |
667/30 | B667 | Per-CP, BB660P2 |
695/40 | B695 | PerCP-Cy5.5, BB700 |
725/40 | B725 | PerCP-eFluor 710 |
Bandpass filter | Parameter | Fluorochrome |
---|---|---|
586/20 | Y586 | PE |
615/20 | Y615 | PE-TexasRed, mCherry, PE-Dazzle 594, PE-CF594, LD Orange |
667/30 | Y667 | PE-Cy5 |
695/40 | Y695 | PE-Cy5.5 |
725/40 | Y725 | PE-AF700 |
780/60 | Y780 | PE-Cy7 |
Bandpass filter | Parameter | Fluorochrome |
---|---|---|
667/30 | R667 | APC, AF647 |
695/40 | R695 | AF680 |
725/40 | R725 | AF700 |
780/60 | R780 | APC-Cy7, APC-H7, APC-Fire750, LDNIR780 |
Location
Sir Alexander Fleming Building, 5th floor, Room 532.
The CellStream is available to any trained user 24 hours a day (subject to building access and regulations).