Analysis software

Registered users at the facility have access to FlowJo as part of our services at no extra cost. Licenses are activated for one week upon request for remote work. Users will need to download the FlowJo software, and we strongly recommend that you read the FlowJo tutorials and watch the FlowJo webinars before attempting any work.

Aurora users are strongly advised to analyse large quantities of data using SpectroFlo, the software specific for Cytek Aurora. The facility has a powerful computer with SpectroFlo installed that can be used as part of our services at no extra cost. The SpectroFlo workstation can be booked via PPMS (link at the bottom of this page) and is located in the Sir Alexander Fleming Building, 5th floor, Room 532. We strongly recommend that you watch the SpectroFlo tutorials on migrating your experiment instance before analysing your data.

An FCS Express license is also available at no extra cost for the Aurora users to analyse raw full spectral cytometry data and, importantly, to overlay the spectra from your single stains for QC purposes (antibody titration, spectra comparison over time and intensity on beads vs cells). The FCS Express workstation can be booked via PPMS (link at the bottom of this page) and is located in the Sir Alexander Fleming Building, 5th floor, Room 532. Users will need to create an account on DeNovo Software first and let us know their sign-in account via email, to activate the license into their accounts before they book the workstation.

Free flow cytometry software packages are another option if you have experience in coding. Many of these free softwares allow you to write tools that are specific for your analysis. The Purdue University Cytometry Laboratories has a comprehensive list. However please note that the facility staff won´t be able to offer assistance on using and coding free software.

More information on data analysis and data presentation can be found in the 'Software, open research and publishing' section of our 'Resources' page.