BD FACSCalibur
The BD FACSCalibur is offered for CL2 work. Please refer to the Biosafety page (link at the bottom of this page) for more information on the documentation needed to use this instrument.
The Calibur is a four colour analyser with two lasers (Blue 488nm and Red 635nm) and a fixed optical alignment. The data acquisition rate is 2,000 events/second. For optimal results we recommend a sample concentration range of 1 x 105 to 2 x 107 particles/mL and a sample volume of < 1 mL.
For more information, please read the BD FACSCalibur Instructions For Use manual.
A more detailed optical configuration including fluorochromes and bandpass filters can be found below.
| Laser | Bandpass Filter | Fluorochrome |
|---|---|---|
| Blue 488nm | 530/30 | FITC, GFP |
| 585/42 | PE, PI | |
| > 670 | PerCP, PerCP-Cy5.5, PE-Cy7 | |
| Red 640nm | 661/16 | APC |
Location
Flowers Building, 3rd Floor, Containment Level 2 Lab 3.3.
The Calibur is available to any trained user 24 hours a day (subject to building access and regulations).
