BD LSRFortessa I
The Fortessa I is a 14 colour analyser with four lasers (Violet 405nm, Blue 488nm, Yellow-Green 561nm and Red 640nm), a fixed optical alignment and a pressure-driven fluidics system. The flow cell is a gel-coupled cuvette as opposed to the jet-in-air system, and this combined with trigon and octagon detection systems provide increased sensitivity and resolution. This arrangement allows filter and mirror changes within the optical array to be made easily and requires no additional alignment for maximum signal strength.
Forward and side scatter properties (FSC and SSC) allow for a minimum detectable particle size of 0.5 μm. The maximum data acquisition rate is 40,000 events/second. We recommend a minimum sample volume of 300 μL.
For more information, please read the BD LSRFortessa Cell Analyzer Technical Specifications.
Download a complete configuration of Fortessa I Lasers and Filters, including fluorochromes and bandpass filters. You can also find it below.
Bandpass filter | Mirror | Fluorochrome |
---|---|---|
450/50 | - | DAPI, Pacific Blue, BV421, Marina Blue, AF405 |
525/50 | 475LP | AmCyan, BV510, Qdot 525 |
605/12 | 595LP | BV605, Qdot 605 |
710/40 | 690LP | BV711 |
Bandpass filter | Mirror | Fluorochrome |
---|---|---|
488/10 | - | - |
530/30 | 505LP | FITC, AF488, GFP |
695/40 | 635LP | PerCP-Cy5.5, Per-CP |
Bandpass filter | Mirror | Fluorochrome |
---|---|---|
582/15 | - | PE, AF546 |
610/20 | 600LP | PE-TexasRe, PE-CF594, mCherry |
660/20 | 630LP | PE-Cy5 |
710/50 | 685LP | PE-AF700, PE-Cy5.5 |
780/60 | 750LP | PE-Cy7 |
Bandpass filter | Mirror | Fluorochrome |
---|---|---|
670/14 | - | APC, AF647 |
730/45 | 710LP | AF700 |
780/60 | 755LP | APC-Cy7, APC-H7 |
Location
Sir Alexander Fleming Building, 5th floor, Room 532.
The Fortessa I is available to any trained user 24 hours a day (subject to building access and regulations).