@article{Malpartida:2023:10.1128/spectrum.05222-22,
author = {Malpartida, Cardenas K and Moser, N and Ansah, F and Pennisi, I and Ahu, Prah D and Eva, Amoah L and Awandare, G and Hafalla, JC and Cunnington, A and Baum, J and Rodriguez, Manzano J and Georgiou, P},
doi = {10.1128/spectrum.05222-22},
journal = {Microbiology Spectrum},
pages = {1--12},
title = {Sensitive detection of asymptomatic and symptomatic malaria with seven novel parasite-specific LAMP assays and translation for use at point-of-care},
url = {http://dx.doi.org/10.1128/spectrum.05222-22},
volume = {11},
year = {2023}
}

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TY  - JOUR
AB  - Human malaria is a life-threatening parasitic disease with high impact in the sub-Saharan Africa region, where 95% of global cases occurred in 2021. While most malaria diagnostic tools are focused on Plasmodium falciparum, there is a current lack of testing non-P. falciparum cases, which may be underreported and, if undiagnosed or untreated, may lead to severe consequences. In this work, seven species-specific loop-mediated isothermal amplification (LAMP) assays were designed and evaluated against TaqMan quantitative PCR (qPCR), microscopy, and enzyme-linked immunosorbent assays (ELISAs). Their clinical performance was assessed with a cohort of 164 samples of symptomatic and asymptomatic patients from Ghana. All asymptomatic samples with a parasite load above 80 genomic DNA (gDNA) copies per μL of extracted sample were detected with the Plasmodium falciparum LAMP assay, reporting 95.6% (95% confidence interval [95% CI] of 89.9 to 98.5) sensitivity and 100% (95% CI of 87.2 to 100) specificity. This assay showed higher sensitivity than microscopy and ELISA, which were 52.7% (95% CI of 39.7 to 67%) and 67.3% (95% CI of 53.3 to 79.3%), respectively. Nine samples were positive for P. malariae, indicating coinfections with P. falciparum, which represented 5.5% of the tested population. No samples were detected as positive for P. vivax, P. ovale, P. knowlesi, or P. cynomolgi by any method. Furthermore, translation to the point-of-care was demonstrated with a subcohort of 18 samples tested locally in Ghana using our handheld lab-on-chip platform, Lacewing, showing comparable results to a conventional fluorescence-based instrument. The developed molecular diagnostic test could detect asymptomatic malaria cases, including submicroscopic parasitemia, and it has the potential to be used for point-of-care applications.
AU  - Malpartida,Cardenas K
AU  - Moser,N
AU  - Ansah,F
AU  - Pennisi,I
AU  - Ahu,Prah D
AU  - Eva,Amoah L
AU  - Awandare,G
AU  - Hafalla,JC
AU  - Cunnington,A
AU  - Baum,J
AU  - Rodriguez,Manzano J
AU  - Georgiou,P
DO  - 10.1128/spectrum.05222-22
EP  - 12
PY  - 2023///
SN  - 2165-0497
SP  - 1
TI  - Sensitive detection of asymptomatic and symptomatic malaria with seven novel parasite-specific LAMP assays and translation for use at point-of-care
T2  - Microbiology Spectrum
UR  - http://dx.doi.org/10.1128/spectrum.05222-22
UR  - https://journals.asm.org/doi/10.1128/spectrum.05222-22
UR  - http://hdl.handle.net/10044/1/103963
VL  - 11
ER  - 

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