CBC student publication selected as a hot article by RSC
The article, featured in Chemical Communication, was written by Doctoral Training Centre student Gillian Busch, and documents the introduction of a chemical tag to track proteins in cells.
Specific N-terminal protein labelling: use of FMDV 3Cpro protease and native chemical ligation
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Gillian K. Busch, Edward W. Tate, Piers R. J. Gaffney, Erika Rosivatz, Rudiger Woscholski and Robin J. Leatherbarrow
Abstract: We report an effective strategy for generating N-terminal cysteinyl proteins by proteolytic cleavage using the enzyme 3Cpro, suitable for a wide range of applications via native chemical ligation.
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