@article{Sreedharan:2017:10.1021/jacs.7b08772,
author = {Sreedharan, S and Gill, MR and Garcia, E and Saeed, HK and Robinson, D and Byrne, A and Cadby, A and Keyes, TE and Smythe, C and Pellett, P and Bernardino, de la Serna J and Thomas, JA},
doi = {10.1021/jacs.7b08772},
journal = {Journal of the American Chemical Society},
pages = {15907--15913},
title = {Multimodal super-resolution optical microscopy using a transition-metal-based probe provides unprecedented capabilities for imaging both nuclear chromatin and mitochondria},
url = {http://dx.doi.org/10.1021/jacs.7b08772},
volume = {139},
year = {2017}
}

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TY  - JOUR
AB  - Detailed studies on the live cell uptake properties of a dinuclear membrane-permeable RuII cell probe show that, at low concentrations, the complex localizes and images mitochondria. At concentrations above ∼20 μM, the complex images nuclear DNA. Because the complex is extremely photostable, has a large Stokes shift, and displays intrinsic subcellular targeting, its compatibility with super-resolution techniques was investigated. It was found to be very well suited to image mitochondria and nuclear chromatin in two color, 2C-SIM, and STED and 3D-STED, both in fixed and live cells. In particular, due to its vastly improved photostability compared to that of conventional SR probes, it can provide images of nuclear DNA at unprecedented resolution.
AU  - Sreedharan,S
AU  - Gill,MR
AU  - Garcia,E
AU  - Saeed,HK
AU  - Robinson,D
AU  - Byrne,A
AU  - Cadby,A
AU  - Keyes,TE
AU  - Smythe,C
AU  - Pellett,P
AU  - Bernardino,de la Serna J
AU  - Thomas,JA
DO  - 10.1021/jacs.7b08772
EP  - 15913
PY  - 2017///
SN  - 0002-7863
SP  - 15907
TI  - Multimodal super-resolution optical microscopy using a transition-metal-based probe provides unprecedented capabilities for imaging both nuclear chromatin and mitochondria
T2  - Journal of the American Chemical Society
UR  - http://dx.doi.org/10.1021/jacs.7b08772
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000415028200052&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/107646
VL  - 139
ER  - 

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