@article{Kolsum:2017:10.1186/s12931-017-0570-5,
author = {Kolsum, U and Donaldson, GC and Singh, R and Barker, BL and Gupta, V and George, L and Webb, AJ and Thurston, S and Brookes, AJ and McHugh, TD and Wedzicha, JA and Brightling, CE and Singh, D},
doi = {10.1186/s12931-017-0570-5},
journal = {Respiratory Research},
title = {Blood and sputum eosinophils in COPD; relationship with bacterial load},
url = {http://dx.doi.org/10.1186/s12931-017-0570-5},
volume = {18},
year = {2017}
}

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TY  - JOUR
AB  - BACKGROUND: Sputum and blood eosinophil counts predict corticosteroid effects in COPD patients. Bacterial infection causes increased airway neutrophilic inflammation. The relationship of eosinophil counts with airway bacterial load in COPD patients is uncertain. We tested the hypothesis that bacterial load and eosinophil counts are inversely related. METHODS: COPD patients were seen at stable state and exacerbation onset. Sputum was processed for quantitative polymerase chain reaction detection of the potentially pathogenic microorganisms (PPM) H. influenzae, M. catarrhalis and S. pneumoniae. PPM positive was defined as total load ≥1 × 10(4)copies/ml. Sputum and whole blood were analysed for differential cell counts. RESULTS: At baseline, bacterial counts were not related to blood eosinophils, but sputum eosinophil % was significantly lower in patients with PPM positive compared to PPM negative samples (medians: 0.5% vs. 1.25% respectively, p = 0.01). Patients with PPM positive samples during an exacerbation had significantly lower blood eosinophil counts at exacerbation compared to baseline (medians: 0.17 × 10(9)/L vs. 0.23 × 10(9)/L respectively, p = 0.008), while no blood eosinophil change was observed with PPM negative samples. CONCLUSIONS: These findings indicate an inverse relationship between bacterial infection and eosinophil counts. Bacterial infection may influence corticosteroid responsiveness by altering the profile of neutrophilic and eosinophilic inflammation.
AU  - Kolsum,U
AU  - Donaldson,GC
AU  - Singh,R
AU  - Barker,BL
AU  - Gupta,V
AU  - George,L
AU  - Webb,AJ
AU  - Thurston,S
AU  - Brookes,AJ
AU  - McHugh,TD
AU  - Wedzicha,JA
AU  - Brightling,CE
AU  - Singh,D
DO  - 10.1186/s12931-017-0570-5
PY  - 2017///
SN  - 1465-993X
TI  - Blood and sputum eosinophils in COPD; relationship with bacterial load
T2  - Respiratory Research
UR  - http://dx.doi.org/10.1186/s12931-017-0570-5
UR  - http://www.ncbi.nlm.nih.gov/pubmed/28482840
UR  - http://hdl.handle.net/10044/1/48924
VL  - 18
ER  - 

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