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@article{Warren:2015:10.3390/ijms160714695,
author = {Warren, SC and Margineanu, A and Katan, M and Dunsby, C and French, PM},
doi = {10.3390/ijms160714695},
journal = {International Journal of Molecular Sciences},
pages = {14695--14716},
title = {Homo-FRET Based Biosensors and Their Application to Multiplexed Imaging of Signalling Events in Live Cells.},
url = {http://dx.doi.org/10.3390/ijms160714695},
volume = {16},
year = {2015}
}
TY - JOUR
AB - Multiplexed imaging of Förster Resonance Energy Transfer (FRET)-based biosensors potentially presents a powerful approach to monitoring the spatio-temporal correlation of signalling pathways within a single live cell. Here, we discuss the potential of homo-FRET based biosensors to facilitate multiplexed imaging. We demonstrate that the homo-FRET between pleckstrin homology domains of Akt (Akt-PH) labelled with mCherry may be used to monitor 3'-phosphoinositide accumulation in live cells and show how global analysis of time resolved fluorescence anisotropy measurements can be used to quantify this accumulation. We further present multiplexed imaging readouts of calcium concentration, using fluorescence lifetime measurements of TN-L15-a CFP/YFP based hetero-FRET calcium biosensor-with 3'-phosphoinositide accumulation.
AU - Warren,SC
AU - Margineanu,A
AU - Katan,M
AU - Dunsby,C
AU - French,PM
DO - 10.3390/ijms160714695
EP - 14716
PY - 2015///
SN - 1661-6596
SP - 14695
TI - Homo-FRET Based Biosensors and Their Application to Multiplexed Imaging of Signalling Events in Live Cells.
T2 - International Journal of Molecular Sciences
UR - http://dx.doi.org/10.3390/ijms160714695
UR - http://hdl.handle.net/10044/1/24650
VL - 16
ER -