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@article{Kwakwa:2016:10.1002/jbio.201500324,
author = {Kwakwa, K and Savell, A and Davies, T and Munro, I and Parrinello, S and Purbhoo, MA and Dunsby, C and Neil, MAA and French, PMW},
doi = {10.1002/jbio.201500324},
journal = {Journal of Biophotonics},
pages = {948--957},
title = {easySTORM: a robust, lower-cost approach to localisation and TIRF microscopy},
url = {http://dx.doi.org/10.1002/jbio.201500324},
volume = {9},
year = {2016}
}
TY - JOUR
AB - TIRF and STORM microscopy are super-resolving fluorescence imaging modalities for which current implementations on standard microscopes can present significant complexity and cost. We present a straightforward and low-cost approach to implement STORM and TIRF taking advantage of multimode optical fibres and multimode diode lasers to provide the required excitation light. Combined with open source software and relatively simple protocols to prepare samples for STORM, including the use of Vectashield for non-TIRF imaging, this approach enables TIRF and STORM imaging of cells labelled with appropriate dyes or expressing suitable fluorescent proteins to become widely accessible at low cost.
AU - Kwakwa,K
AU - Savell,A
AU - Davies,T
AU - Munro,I
AU - Parrinello,S
AU - Purbhoo,MA
AU - Dunsby,C
AU - Neil,MAA
AU - French,PMW
DO - 10.1002/jbio.201500324
EP - 957
PY - 2016///
SN - 1864-0648
SP - 948
TI - easySTORM: a robust, lower-cost approach to localisation and TIRF microscopy
T2 - Journal of Biophotonics
UR - http://dx.doi.org/10.1002/jbio.201500324
UR - http://hdl.handle.net/10044/1/29174
VL - 9
ER -