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@article{Maioli:2016:10.1038/srep37777,
author = {Maioli, V and Chennell, G and Sparks, H and Lana, T and Kumar, S and Carling, D and Sardini, A and Dunsby, CW},
doi = {10.1038/srep37777},
journal = {Scientific Reports},
title = {Time-lapse 3-D measurements of a glucose biosensor in multicellular spheroids by light sheet fluorescence microscopy in commercial 96-well plates},
url = {http://dx.doi.org/10.1038/srep37777},
volume = {6},
year = {2016}
}
TY - JOUR
AB - Light sheet fluorescence microscopy has previously been demonstrated on a commercially available inverted fluorescence microscope frame using the method of oblique plane microscopy (OPM). In this paper, OPM is adapted to allow time-lapse 3-D imaging of 3-D biological cultures in commercially available glass-bottomed 96-well plates using a stage-scanning OPM approach (ssOPM). Time-lapse 3-D imaging of multicellular spheroids expressing a glucose Förster resonance energy transfer (FRET) biosensor is demonstrated in 16 fields of view with image acquisition at 10 minute intervals. As a proof-of-principle, the ssOPM system is also used to acquire a dose response curve with the concentration of glucose in the culture medium being varied across 42 wells of a 96-well plate with the whole acquisition taking 9 min. The 3-D image data enable the FRET ratio to be measured as a function of distance from the surface of the spheroid. Overall, the results demonstrate the capability of the OPM system to measure spatio-temporal changes in FRET ratio in 3-D in multicellular spheroids over time in a multi-well plate format.
AU - Maioli,V
AU - Chennell,G
AU - Sparks,H
AU - Lana,T
AU - Kumar,S
AU - Carling,D
AU - Sardini,A
AU - Dunsby,CW
DO - 10.1038/srep37777
PY - 2016///
SN - 2045-2322
TI - Time-lapse 3-D measurements of a glucose biosensor in multicellular spheroids by light sheet fluorescence microscopy in commercial 96-well plates
T2 - Scientific Reports
UR - http://dx.doi.org/10.1038/srep37777
UR - http://hdl.handle.net/10044/1/42274
VL - 6
ER -