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@article{Sparks:2017:10.1063/1.4973917,
author = {Sparks, H and Gorlitz, F and Kelly, D and Warren, SC and Kellet, PA and Garcia, E and Dymoke-Bradshaw, AKL and Hares, JD and Neil, MAA and Dunsby, C and French, PMW},
doi = {10.1063/1.4973917},
journal = {Review of Scientific Instruments},
title = {Characterisation of new gated optical image intensifiers for fluorescence lifetime imaging},
url = {http://dx.doi.org/10.1063/1.4973917},
volume = {88},
year = {2017}
}
TY - JOUR
AB - We report the characterisation of gated optical image intensifiers for fluorescence lifetime imaging (FLIM), evaluating the performance of several different prototypes that culminate in a new design that provides improved spatial resolution conferred by the addition of a magnetic field to reduce the lateral spread of photoelectrons on their path between the photocathode and microchannel plate, and higher signal to noise ratio conferred by longer time gates. We also present a methodology to compare thesesystems and their capabilities, including the quantitative readouts of Förster resonant energy transfer (FRET).
AU - Sparks,H
AU - Gorlitz,F
AU - Kelly,D
AU - Warren,SC
AU - Kellet,PA
AU - Garcia,E
AU - Dymoke-Bradshaw,AKL
AU - Hares,JD
AU - Neil,MAA
AU - Dunsby,C
AU - French,PMW
DO - 10.1063/1.4973917
PY - 2017///
SN - 1089-7623
TI - Characterisation of new gated optical image intensifiers for fluorescence lifetime imaging
T2 - Review of Scientific Instruments
UR - http://dx.doi.org/10.1063/1.4973917
UR - http://hdl.handle.net/10044/1/43748
VL - 88
ER -