@article{Pellegrino:2022:10.1021/acsami.2c08768,
author = {Pellegrino, L and Kriem, LS and Robles, ESJ and Cabral, JT},
doi = {10.1021/acsami.2c08768},
journal = {ACS Applied Materials and Interfaces},
title = {Microbial response to micrometer-scale multiaxial wrinkled surfaces.},
url = {http://dx.doi.org/10.1021/acsami.2c08768},
volume = {14},
year = {2022}
}

Download

TY  - JOUR
AB  - We investigate the effect of micrometer-scale surface wrinkling on the attachment and proliferation of model bacteria (Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli K12) and fungi (Candida albicans). Specifically, sinusoidal (1D), checkerboard (C), and herringbone (H) patterns were fabricated by mechanical wrinkling of plasma-oxidized polydimethylsiloxane (PDMS) bilayers and contrasted with flat (F) surfaces. Microbial deformation and orientation were found to correlate with the aspect ratio and commensurably with surface pattern dimensions and local pattern order. Significantly, the proliferation of P. aeruginosa could be described by a linear scaling between bacterial area coverage and available surface area, defined as a fraction of the line integral along each profile with negative curvature. However, in the early stages of proliferation (up to 6 h examined), that C and H patterns disrupt the spatial arrangement of bacteria, impeding proliferation for several hours and reducing it (by ∼50%) thereafter. Our findings suggest a simple framework to rationalize the impact of micrometer-scale topography on microbial action and demonstrate that multiaxial patterning order provides an effective strategy to delay and frustrate the early stages of bacterial proliferation.
AU  - Pellegrino,L
AU  - Kriem,LS
AU  - Robles,ESJ
AU  - Cabral,JT
DO  - 10.1021/acsami.2c08768
PY  - 2022///
SN  - 1944-8244
TI  - Microbial response to micrometer-scale multiaxial wrinkled surfaces.
T2  - ACS Applied Materials and Interfaces
UR  - http://dx.doi.org/10.1021/acsami.2c08768
UR  - https://www.ncbi.nlm.nih.gov/pubmed/35699282
UR  - https://pubs.acs.org/doi/10.1021/acsami.2c08768
UR  - http://hdl.handle.net/10044/1/99524
VL  - 14
ER  - 

Download