In this section
@article{Rowlands:2017:10.1038/lsa.2016.255,
author = {Rowlands, CJ and Park, D and Bruns, OT and Piatkevich, KD and Fukumura, D and Jain, RK and Bawendi, MG and Boyden, ES and So, PTC},
doi = {10.1038/lsa.2016.255},
journal = {Light: Science and Applications},
pages = {1--9},
title = {Wide-field three-photon excitation in biological samples},
url = {http://dx.doi.org/10.1038/lsa.2016.255},
volume = {6},
year = {2017}
}
TY - JOUR
AB - Three-photon wide-field depth-resolved excitation is used to overcome some of the limitations in conventional point-scanning two- and three-photon microscopy. Excitation of chromophores as diverse as channelrhodopsins and quantum dots is shown, and a penetration depth of more than 700 μm into fixed scattering brain tissue is achieved, approximately twice as deep as that achieved using two-photon wide-field excitation. Compatibility with live animal experiments is confirmed by imaging the cerebral vasculature of an anesthetized mouse; a complete focal stack was obtained without any evidence of photodamage. As an additional validation of the utility of wide-field three-photon excitation, functional excitation is demonstrated by performing three-photon optogenetic stimulation of cultured mouse hippocampal neurons expressing a channelrhodopsin; action potentials could reliably be excited without causing photodamage.
AU - Rowlands,CJ
AU - Park,D
AU - Bruns,OT
AU - Piatkevich,KD
AU - Fukumura,D
AU - Jain,RK
AU - Bawendi,MG
AU - Boyden,ES
AU - So,PTC
DO - 10.1038/lsa.2016.255
EP - 9
PY - 2017///
SN - 2047-7538
SP - 1
TI - Wide-field three-photon excitation in biological samples
T2 - Light: Science and Applications
UR - http://dx.doi.org/10.1038/lsa.2016.255
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000402396000005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - https://www.nature.com/articles/lsa2016255
VL - 6
ER -
Email: c.rowlands@imperial.ac.uk
Telephone: +44 (0)20 7594 1331
Department of Bioengineering
Royal School of Mines
Imperial College London
SW7 2AS