BibTex format
@article{Kientega:2024:10.1186/s12936-024-05106-7,
author = {Kientega, M and Clarkson, CS and Traoré, N and Hui, T-YJ and O'Loughlin, S and Millogo, A-A and Epopa, PS and Yao, FA and Belem, AMG and Brenas, J and Miles, A and Burt, A and Diabaté, A},
doi = {10.1186/s12936-024-05106-7},
journal = {Malaria Journal},
title = {Whole-genome sequencing of major malaria vectors reveals the evolution of new insecticide resistance variants in a longitudinal study in Burkina Faso},
url = {http://dx.doi.org/10.1186/s12936-024-05106-7},
volume = {23},
year = {2024}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - BACKGROUND: Intensive deployment of insecticide based malaria vector control tools resulted in the rapid evolution of phenotypes resistant to these chemicals. Understanding this process at the genomic level is important for the deployment of successful vector control interventions. Therefore, longitudinal sampling followed by whole genome sequencing (WGS) is necessary to understand how these evolutionary processes evolve over time. This study investigated the change in genetic structure and the evolution of the insecticide resistance variants in natural populations of Anopheles gambiae over time and space from 2012 to 2017 in Burkina Faso. METHODS: New genomic data have been generated from An. gambiae mosquitoes collected from three villages in the western part of Burkina Faso between 2012 and 2017. The samples were whole-genome sequenced and the data used in the An. gambiae 1000 genomes (Ag1000G) project as part of the Vector Observatory. Genomic data were analysed using the analysis pipeline previously designed by the Ag1000G project. RESULTS: The results showed similar and consistent nucleotide diversity and negative Tajima's D between An. gambiae sensu stricto (s.s.) and Anopheles coluzzii. Principal component analysis (PCA) and the fixation index (FST) showed a clear genetic structure in the An. gambiae sensu lato (s.l.) species. Genome-wide FST and H12 scans identified genomic regions under divergent selection that may have implications in the adaptation to ecological changes. Novel voltage-gated sodium channel pyrethroid resistance target-site alleles (V402L, I1527T) were identified at increasing frequencies alongside the established alleles (Vgsc-L995F, Vgsc-L995S and N1570Y) within the An. gambiae s.l. POPULATIONS: Organophosphate metabolic resistance markers were also identified, at increasing frequencies, within the An. gambiae s.s. populations from 2012 to 2017, including the SNP Ace1-G280S and its associated duplication. Variants simultaneously identifi
AU - Kientega,M
AU - Clarkson,CS
AU - Traoré,N
AU - Hui,T-YJ
AU - O'Loughlin,S
AU - Millogo,A-A
AU - Epopa,PS
AU - Yao,FA
AU - Belem,AMG
AU - Brenas,J
AU - Miles,A
AU - Burt,A
AU - Diabaté,A
DO - 10.1186/s12936-024-05106-7
PY - 2024///
SN - 1475-2875
TI - Whole-genome sequencing of major malaria vectors reveals the evolution of new insecticide resistance variants in a longitudinal study in Burkina Faso
T2 - Malaria Journal
UR - http://dx.doi.org/10.1186/s12936-024-05106-7
UR - https://www.ncbi.nlm.nih.gov/pubmed/39285410
UR - https://malariajournal.biomedcentral.com/articles/10.1186/s12936-024-05106-7
UR - http://hdl.handle.net/10044/1/114700
VL - 23
ER -