@article{Alagaratnam:2024:10.1016/j.jve.2024.100381,
author = {Alagaratnam, J and Stöhr, W and Hamlyn, E and Porter, K and Toombs, J and Heslegrave, A and Zetterberg, H and Gisslén, M and Underwood, J and Schechter, M and Kaleebu, P and Tambussi, G and Kinloch, S and Miro, JM and Kelleher, AD and Babiker, A and Frater, J and Winston, A and Fidler, S},
doi = {10.1016/j.jve.2024.100381},
journal = {Journal of Virus Eradication},
title = {Impact of interrupting antiretroviral therapy started during primary HIV-1 infection on plasma neurofilament light chain protein, a marker of neuronal injury: the SPARTAC trial},
url = {http://dx.doi.org/10.1016/j.jve.2024.100381},
volume = {10},
year = {2024}
}

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TY  - JOUR
AB  - ObjectiveAntiretroviral therapy (ART)-conferred suppression of HIV replication limits neuronal injury and inflammation. ART interruption tests efficacy in HIV cure trials and viral rebound after ART interruption may induce neuronal injury. We investigated the impact of protocol-defined ART interruption, commenced during primary HIV-1 infection (PHI) on a biomarker of neuro-axonal injury (neurofilament light protein (NfL)), and its associations with inflammation (D-dimer and interleukin-6 (IL-6)) and HIV-1 reservoir size (total HIV-1 DNA).DesignRetrospective study measuring plasma NfL in 83 participants enrolled in SPARTAC randomised to receive 48-weeks ART initiated during PHI, followed by ART interruption.MethodsNfL (Simoa immunoassay, Quanterix™) was measured before ART, after 48 weeks on ART, and 12 weeks after stopping ART. Plasma D-dimer and IL-6, and total HIV-1 DNA in peripheral CD4+ T-cells results were available in a subset of participants. Longitudinal NfL changes were assessed using mixed models, and associations with clinical and laboratory parameters using linear regression.ResultsNfL decreased following 48-weeks ART (geometric mean 6.9 to 5.8 pg/mL, p = 0.006) with no further significant change up to 12-weeks post-stopping ART despite viral rebound in the majority of participants (median 1.7 to 3.9 plasma HIV-1 RNA log10 copies/mL). Higher baseline NfL was independently associated with higher plasma HIV-1 RNA (p = 0.020) and older age (p = 0.002). While NfL was positively associated with D-dimer (n = 48; p = 0.002), there was no significant association with IL-6 (n = 48) or total HIV-1 DNA (n = 51).ConclusionsUsing plasma NfL as a surrogate marker, a decrease in neuro-axonal injury was observed in a cohort of participants following ART initiation during PHI, with no evidence of neuro-axonal injury rebound following ART interruption for up to 12 weeks, despite viral rebound in the majority of participants.
AU  - Alagaratnam,J
AU  - Stöhr,W
AU  - Hamlyn,E
AU  - Porter,K
AU  - Toombs,J
AU  - Heslegrave,A
AU  - Zetterberg,H
AU  - Gisslén,M
AU  - Underwood,J
AU  - Schechter,M
AU  - Kaleebu,P
AU  - Tambussi,G
AU  - Kinloch,S
AU  - Miro,JM
AU  - Kelleher,AD
AU  - Babiker,A
AU  - Frater,J
AU  - Winston,A
AU  - Fidler,S
DO  - 10.1016/j.jve.2024.100381
PY  - 2024///
SN  - 2055-6640
TI  - Impact of interrupting antiretroviral therapy started during primary HIV-1 infection on plasma neurofilament light chain protein, a marker of neuronal injury: the SPARTAC trial
T2  - Journal of Virus Eradication
UR  - http://dx.doi.org/10.1016/j.jve.2024.100381
UR  - http://hdl.handle.net/10044/1/112636
VL  - 10
ER  - 

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