In this section
@article{Seong:2020:10.1021/acsnano.9b08689,
author = {Seong, H and Higgins, SG and Penders, J and Armstrong, JPK and Crowder, SW and Moore, AC and Sero, JE and Becce, M and Stevens, MM},
doi = {10.1021/acsnano.9b08689},
journal = {ACS Nano},
pages = {5371--5381},
title = {Size-tunable nanoneedle arrays for influencing stem cell morphology, gene expression and nuclear membrane curvature},
url = {http://dx.doi.org/10.1021/acsnano.9b08689},
volume = {14},
year = {2020}
}
TY - JOUR
AB - High-aspect-ratio nanostructures have emerged as versatile platforms for intracellular sensing and biomolecule delivery. Here, we present a microfabrication approach in which a combination of reactive ion etching protocols was used to produce high-aspect-ratio, nondegradable silicon nanoneedle arrays with tip diameters that can be finely tuned between 20 and 700 nm. We used these arrays to guide the long-term culture of human mesenchymal stem cells (hMSCs). Notably, we used the nanoneedle tip diameter to control the morphology, nuclear size and F-actin alignment of interfaced hMSCs, and to regulate the expression of nuclear lamina genes, Yes-associated protein (YAP) target genes and focal adhesion genes. These topography-driven changes were attributed to signaling by Rho-family GTPase pathways, differences in the effective stiffness of the nanoneedle arrays and the degree of nuclear membrane impingement, with the latter clearly visualized using focused-ion beam scanning electron microscopy (FIB-SEM). Our approach to design high-aspect-ratio nanostructures will be broadly applicable to design biomaterials and biomedical devices used for long-term cell stimulation and monitoring.
AU - Seong,H
AU - Higgins,SG
AU - Penders,J
AU - Armstrong,JPK
AU - Crowder,SW
AU - Moore,AC
AU - Sero,JE
AU - Becce,M
AU - Stevens,MM
DO - 10.1021/acsnano.9b08689
EP - 5381
PY - 2020///
SN - 1936-0851
SP - 5371
TI - Size-tunable nanoneedle arrays for influencing stem cell morphology, gene expression and nuclear membrane curvature
T2 - ACS Nano
UR - http://dx.doi.org/10.1021/acsnano.9b08689
UR - https://pubs.acs.org/doi/10.1021/acsnano.9b08689
UR - http://hdl.handle.net/10044/1/78532
VL - 14
ER -
If you would like to become a member of the network please contact us.
Network Lead
Dr Michela Noseda
Network Lead
Dr Blerina Ahmetaj-Shala
Network Coordinator
Zuzanna Jablonska
Network Coordinator
Marine Secchi
General enquiries
ISRMNcontact@imperial.ac.uk
