@article{Machado:2017:10.1016/j.jeurceramsoc.2017.06.043,
author = {Machado, GC and García-Tuñón, E and Bell, RV and Alini, M and Saiz, E and Peroglio, M},
doi = {10.1016/j.jeurceramsoc.2017.06.043},
journal = {Journal of the European Ceramic Society},
pages = {949--961},
title = {Calcium phosphate substrates with emulsion-derived roughness: processing, characterisation and interaction with human mesenchymal stem cells},
url = {http://dx.doi.org/10.1016/j.jeurceramsoc.2017.06.043},
volume = {38},
year = {2017}
}

Download

TY  - JOUR
AB  - Calcium phosphates (CaP) have been the subject of several studies that often lack a systematic approach to understanding how their properties affect biological response. CaP particles functionalised with a pH-responsive polymer (BCS) were used to prepare microporous substrates (porosity between 70 and 75% and pore sizes of 5–20 μm) through the aggregation of oil-in-water emulsions by controlling solid loading, emulsification energy, pH, drying and sintering conditions. The combined effect of surface roughness (roughness amplitude, Ra between 0.9–1.7 μm) and chemistry (varying Hydroxyapatite/β-Tricalcium phosphate ratio) on human mesenchymal stem cells was evaluated. HA substrates stimulated higher cell adhesion and proliferation (especially with lower Ra), but cell area increased with β-TCP content. The effect of surface roughness depended of chemistry: HA promoted higher mineralising activity when Ra ∼ 1.5 μm, whereas β-TCP substrates stimulated a more osteogenic profile when Ra ∼ 1.7 μm. A novel templating method to fabricate microporous CaP substrates was developed, opening possibilities for bone substitutes with controlled features.
AU  - Machado,GC
AU  - García-Tuñón,E
AU  - Bell,RV
AU  - Alini,M
AU  - Saiz,E
AU  - Peroglio,M
DO  - 10.1016/j.jeurceramsoc.2017.06.043
EP  - 961
PY  - 2017///
SN  - 0955-2219
SP  - 949
TI  - Calcium phosphate substrates with emulsion-derived roughness: processing, characterisation and interaction with human mesenchymal stem cells
T2  - Journal of the European Ceramic Society
UR  - http://dx.doi.org/10.1016/j.jeurceramsoc.2017.06.043
UR  - http://hdl.handle.net/10044/1/49364
VL  - 38
ER  - 

Download