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@article{Anderson:2020:10.15252/msb.20209475,
author = {Anderson, DP and Benns, HJ and Tate, EW and Child, MA},
doi = {10.15252/msb.20209475},
journal = {Mol Syst Biol},
title = {CRISPR-TAPE: protein-centric CRISPR guide design for targeted proteome engineering.},
url = {http://dx.doi.org/10.15252/msb.20209475},
volume = {16},
year = {2020}
}
TY - JOUR
AB - Rational molecular engineering of proteins with CRISPR-based approaches is challenged by the gene-centric nature of gRNA design tools. To address this, we have developed CRISPR-TAPE, a protein-centric gRNA design algorithm that allows users to target specific residues, or amino acid types within proteins. gRNA outputs can be customized to support maximal efficacy of homology-directed repair for engineering purposes, removing time-consuming post hoc curation, simplifying gRNA outputs and reducing CPU times.
AU - Anderson,DP
AU - Benns,HJ
AU - Tate,EW
AU - Child,MA
DO - 10.15252/msb.20209475
PY - 2020///
TI - CRISPR-TAPE: protein-centric CRISPR guide design for targeted proteome engineering.
T2 - Mol Syst Biol
UR - http://dx.doi.org/10.15252/msb.20209475
UR - https://www.ncbi.nlm.nih.gov/pubmed/32485092
VL - 16
ER -
Prof. Ed Tate
GSK Chair in Chemical Biology
Department of Chemistry
Molecular Sciences Research Hub, White City Campus,
82 Wood Lane, London, W12 0BZ
e.tate@imperial.ac.uk
Tel: +44 (0)20 759 + ext 43752 or 45821