BibTex format
@article{Huang:2023:10.1016/j.molcel.2023.09.004,
author = {Huang, X and Yao, J and Liu, L and Chen, J and Mei, L and Huangfu, J and Luo, D and Wang, X and Lin, C and Chen, X and Yang, Y and Ouyang, S and Wei, F and Wang, Z and Zhang, S and Xiang, T and Neculai, D and Sun, Q and Kong, E and Tate, EW and Yang, A},
doi = {10.1016/j.molcel.2023.09.004},
journal = {Molecular Cell},
pages = {3485--3501.E11},
title = {S-acylation of p62 promotes p62 droplet recruitment into autophagosomes in mammalian autophagy},
url = {http://dx.doi.org/10.1016/j.molcel.2023.09.004},
volume = {83},
year = {2023}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - p62 is a well-characterized autophagy receptor that recognizes and sequesters specific cargoes into autophagosomes for degradation. p62 promotes the assembly and removal of ubiquitinated proteins by forming p62-liquid droplets. However, it remains unclear how autophagosomes efficiently sequester p62 droplets. Herein, we report that p62 undergoes reversible S-acylation in multiple human-, rat-, and mouse-derived cell lines, catalyzed by zinc-finger Asp-His-His-Cys S-acyltransferase 19 (ZDHHC19) and deacylated by acyl protein thioesterase 1 (APT1). S-acylation of p62 enhances the affinity of p62 for microtubule-associated protein 1 light chain 3 (LC3)-positive membranes and promotes autophagic membrane localization of p62 droplets, thereby leading to the production of small LC3-positive p62 droplets and efficient autophagic degradation of p62-cargo complexes. Specifically, increasing p62 acylation by upregulating ZDHHC19 or by genetic knockout of APT1 accelerates p62 degradation and p62-mediated autophagic clearance of ubiquitinated proteins. Thus, the protein S-acylation-deacylation cycle regulates p62 droplet recruitment to the autophagic membrane and selective autophagic flux, thereby contributing to the control of selective autophagic clearance of ubiquitinated proteins.
AU - Huang,X
AU - Yao,J
AU - Liu,L
AU - Chen,J
AU - Mei,L
AU - Huangfu,J
AU - Luo,D
AU - Wang,X
AU - Lin,C
AU - Chen,X
AU - Yang,Y
AU - Ouyang,S
AU - Wei,F
AU - Wang,Z
AU - Zhang,S
AU - Xiang,T
AU - Neculai,D
AU - Sun,Q
AU - Kong,E
AU - Tate,EW
AU - Yang,A
DO - 10.1016/j.molcel.2023.09.004
EP - 3501
PY - 2023///
SN - 1097-2765
SP - 3485
TI - S-acylation of p62 promotes p62 droplet recruitment into autophagosomes in mammalian autophagy
T2 - Molecular Cell
UR - http://dx.doi.org/10.1016/j.molcel.2023.09.004
UR - https://doi.org/10.1016/j.molcel.2023.09.004
UR - http://hdl.handle.net/10044/1/106481
VL - 83
ER -
