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@article{Draganov:2024:10.1039/D4CB00043A,
author = {Draganov, S and Gruet, M and Conole, D and Balcells, C and Siskos, A and Keun, H and Haskard, D and Tate, E},
doi = {10.1039/D4CB00043A},
journal = {RSC Chemical Biology},
pages = {640--651},
title = {Chemical tools for profiling the intracellular ADP-ribosylated proteome},
url = {http://dx.doi.org/10.1039/D4CB00043A},
volume = {5},
year = {2024}
}
TY - JOUR
AB - The post-translational modification (PTM) ADP-ribosylation plays an important role in cell signalling and regulating protein function and has been implicated in the development of multiple diseases, including breast and ovarian cancers. Studying the underlying mechanisms through which this PTM contributes towards disease development, however, has been hampered by the lack of appropriate tools for reliable identification of physiologically relevant ADP-ribosylated proteins in a live-cell environment. Herein, we explore the application of an alkyne-tagged proprobe, 6Yn-ProTide-Ad (6Yn-Pro) as a chemical tool for the identification of intracellular ADP-ribosylated proteins through metabolic labelling. We applied targeted metabolomics and chemical proteomics in HEK293T cells treated with 6Yn-Pro to demonstrate intracellular metabolic conversion of the probe into ADP-ribosylation cofactor 6Yn-NAD+, and subsequent labelling and enrichment of PARP1 and multiple known ADP-ribosylated proteins in cells under hydrogen peroxide-induced stress. We anticipate that the approach and methodology described here will be useful for future identification of novel intracellular ADP-ribosylated proteins.
AU - Draganov,S
AU - Gruet,M
AU - Conole,D
AU - Balcells,C
AU - Siskos,A
AU - Keun,H
AU - Haskard,D
AU - Tate,E
DO - 10.1039/D4CB00043A
EP - 651
PY - 2024///
SN - 2633-0679
SP - 640
TI - Chemical tools for profiling the intracellular ADP-ribosylated proteome
T2 - RSC Chemical Biology
UR - http://dx.doi.org/10.1039/D4CB00043A
UR - https://pubs.rsc.org/en/content/articlelanding/2024/cb/d4cb00043a
UR - http://hdl.handle.net/10044/1/111789
VL - 5
ER -
Prof. Ed Tate
GSK Chair in Chemical Biology
Department of Chemistry
Molecular Sciences Research Hub, White City Campus,
82 Wood Lane, London, W12 0BZ
e.tate@imperial.ac.uk
Tel: +44 (0)20 759 + ext 43752 or 45821