BibTex format
@article{Guttery:2014:10.1016/j.chom.2014.05.020,
author = {Guttery, DS and Poulin, B and Ramaprasad, A and Wall, RJ and Ferguson, DJP and Brady, D and Patzewitz, E-M and Whipple, S and Straschil, U and Wright, MH and Mohamed, AMAH and Radhakrishnan, A and Arold, ST and Tate, EW and Holder, AA and Wickstead, B and Pain, A and Tewari, R},
doi = {10.1016/j.chom.2014.05.020},
journal = {Cell Host & Microbe},
pages = {128--140},
title = {Genome-wide Functional Analysis of Plasmodium Protein Phosphatases Reveals Key Regulators of Parasite Development and Differentiation},
url = {http://dx.doi.org/10.1016/j.chom.2014.05.020},
volume = {16},
year = {2014}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Reversible protein phosphorylation regulated by kinasesand phosphatases controls many cellular processes.Although essential functions for the malariaparasite kinome have been reported, the roles ofmost protein phosphatases (PPs) during Plasmodiumdevelopment are unknown. We report a functionalanalysis of the Plasmodium berghei protein phosphatome,which exhibits high conservation with theP. falciparum phosphatome and comprises 30 predictedPPs with differential and distinct expressionpatterns during various stages of the life cycle. Genedisruption analysis of P. berghei PPs reveals thathalf of the genes are likely essential for asexualblood stage development, whereas six are requiredfor sexual development/sporogony in mosquitoes.Phenotypic screening coupled with transcriptomesequencing unveiled morphological changes andaltered gene expression in deletion mutants of twoN-myristoylated PPs. These findings provide systematicfunctional analyses of PPs in Plasmodium, identifyhow phosphatases regulate parasite developmentand differentiation, and can inform the identification ofdrug targets for malaria.
AU - Guttery,DS
AU - Poulin,B
AU - Ramaprasad,A
AU - Wall,RJ
AU - Ferguson,DJP
AU - Brady,D
AU - Patzewitz,E-M
AU - Whipple,S
AU - Straschil,U
AU - Wright,MH
AU - Mohamed,AMAH
AU - Radhakrishnan,A
AU - Arold,ST
AU - Tate,EW
AU - Holder,AA
AU - Wickstead,B
AU - Pain,A
AU - Tewari,R
DO - 10.1016/j.chom.2014.05.020
EP - 140
PY - 2014///
SN - 1934-6069
SP - 128
TI - Genome-wide Functional Analysis of Plasmodium Protein Phosphatases Reveals Key Regulators of Parasite Development and Differentiation
T2 - Cell Host & Microbe
UR - http://dx.doi.org/10.1016/j.chom.2014.05.020
UR - http://hdl.handle.net/10044/1/26921
VL - 16
ER -
