@article{Marques:2016:nar/gkw147,
author = {Marques, CA and Tiengwe, C and Lemgruber, L and Damasceno, JD and Scott, A and Paape, D and Marcello, L and McCulloch, R},
doi = {nar/gkw147},
journal = {Nucleic Acids Research},
pages = {4763--4784},
title = {Diverged composition and regulation of the Trypanosoma brucei origin recognition complex that mediates DNA replication initiation},
url = {http://dx.doi.org/10.1093/nar/gkw147},
volume = {44},
year = {2016}
}
TY - JOUR
AB - Initiation of DNA replication depends upon recog-nition of genomic sites, termed origins, by AAA+ATPases. In prokaryotes a single factor binds eachorigin, whereas in eukaryotes this role is playedby a six-protein origin recognition complex (ORC).Why eukaryotes evolved a multisubunit initiator, andthe roles of each component, remains unclear. InTrypanosoma brucei, an ancient unicellular eukary-ote, only one ORC-related initiator, TbORC1/CDC6,has been identified by sequence homology. Herewe show that three TbORC1/CDC6-interacting fac-tors also act inT. bruceinuclear DNA replicationand demonstrate that TbORC1/CDC6 interacts in ahigh molecular complex in which a diverged Orc4homologue and one replicative helicase subunit canalso be found. Analysing the subcellular localizationof four TbORC1/CDC6-interacting factors during thecell cycle reveals that one factor, TbORC1B, is nota static constituent of ORC but displays S-phaserestricted nuclear localization and expression, sug-gesting it positively regulates replication. This workshows that ORC architecture and regulation are di-verged features of DNA replication initiation inT. bru-cei, providing new insight into this key stage of eu-karyotic genome copying.
AU - Marques,CA
AU - Tiengwe,C
AU - Lemgruber,L
AU - Damasceno,JD
AU - Scott,A
AU - Paape,D
AU - Marcello,L
AU - McCulloch,R
DO - nar/gkw147
EP - 4784
PY - 2016///
SN - 0305-1048
SP - 4763
TI - Diverged composition and regulation of the Trypanosoma brucei origin recognition complex that mediates DNA replication initiation
T2 - Nucleic Acids Research
UR - http://dx.doi.org/10.1093/nar/gkw147
UR - http://hdl.handle.net/10044/1/59375
VL - 44
ER -