@article{Wincott:2022:10.1016/j.crmeth.2022.100274,
author = {Wincott, CJ and Sritharan, G and Benns, HJ and May, D and Gilabert-Carbajo, C and Bunyan, M and Fairweather, AR and Alves, E and Andrew, I and Game, L and Frickel, E-M and Tiengwe, C and Ewald, SE and Child, MA},
doi = {10.1016/j.crmeth.2022.100274},
journal = {Cell Reports Methods},
pages = {1--16},
title = {Cellular barcoding of protozoan pathogens reveals the within-host population dynamics of Toxoplasma gondii host colonization},
url = {http://dx.doi.org/10.1016/j.crmeth.2022.100274},
volume = {2},
year = {2022}
}

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TY  - JOUR
AB  - Cellular barcoding techniques are powerful tools to understand microbial pathogenesis. However, barcoding strategies have not been broadly applied to protozoan parasites, which have unique genomic structures and virulence strategies compared with viral and bacterial pathogens. Here, we present a CRISPR-based method to barcode protozoa, which we successfully apply to Toxoplasma gondii and Trypanosoma brucei. Using libraries of barcoded T. gondii, we evaluate shifts in the population structure from acute to chronic infection of mice. Contrary to expectation, most barcodes were present in the brain one month post-intraperitoneal infection in both inbred CBA/J and outbred Swiss mice. Although parasite cyst number and barcode diversity declined over time, barcodes representing a minor fraction of the inoculum could become a dominant population in the brain by three months post-infection. These data establish a cellular barcoding approach for protozoa and evidence that the blood-brain barrier is not a major bottleneck to colonization by T. gondii.
AU  - Wincott,CJ
AU  - Sritharan,G
AU  - Benns,HJ
AU  - May,D
AU  - Gilabert-Carbajo,C
AU  - Bunyan,M
AU  - Fairweather,AR
AU  - Alves,E
AU  - Andrew,I
AU  - Game,L
AU  - Frickel,E-M
AU  - Tiengwe,C
AU  - Ewald,SE
AU  - Child,MA
DO  - 10.1016/j.crmeth.2022.100274
EP  - 16
PY  - 2022///
SN  - 2667-2375
SP  - 1
TI  - Cellular barcoding of protozoan pathogens reveals the within-host population dynamics of Toxoplasma gondii host colonization
T2  - Cell Reports Methods
UR  - http://dx.doi.org/10.1016/j.crmeth.2022.100274
UR  - https://www.sciencedirect.com/science/article/pii/S2667237522001515?via%3Dihub
UR  - http://hdl.handle.net/10044/1/99220
VL  - 2
ER  - 

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