In this section
@article{Nahar:2016:jat/bkv125,
author = {Nahar, LK and Cordero, R and Nutt, D and Lingford-Hughes, A and Turton, S and Durant, C and Wilson, S and Paterson, S},
doi = {jat/bkv125},
journal = {Journal of Analytical Toxicology},
pages = {117--123},
title = {Validated method for the quantification of baclofen in human plasma using solid-phase extraction and liquid chromatography–tandem mass spectrometry},
url = {http://dx.doi.org/10.1093/jat/bkv125},
volume = {40},
year = {2016}
}
TY - JOUR
AB - A highly sensitive and fully validated method was developed for the quantification of baclofen in human plasma. After adjusting the pH of the plasma samples using a phosphate buffer solution (pH 4), baclofen was purified using mixed mode (C8/cation exchange) solid-phase extraction (SPE) cartridges. Endogenous water-soluble compounds and lipids were removed from the cartridges before the samples were eluted and concentrated. The samples were analyzed using triple-quadrupoleliquid chromatography–tandem mass spectrometry (LC–MS-MS) with triggered dynamic multiple reaction monitoring mode for simultaneous quantification and confirmation. The assay was linear from 25 to 1,000 ng/mL (r2 > 0.999; n = 6). Intraday (n = 6) and interday (n = 15) imprecisions (% relative standard deviation) were <5%, and the average recovery was 30%. The limit of detection of the method was 5 ng/mL, and the limit of quantification was 25 ng/mL. Plasma samples from healthymale volunteers (n = 9, median age: 22) given two single oral doses of baclofen (10 and 60 mg) on nonconsecutive days were analyzed to demonstrate method applicability.
AU - Nahar,LK
AU - Cordero,R
AU - Nutt,D
AU - Lingford-Hughes,A
AU - Turton,S
AU - Durant,C
AU - Wilson,S
AU - Paterson,S
DO - jat/bkv125
EP - 123
PY - 2016///
SN - 0146-4760
SP - 117
TI - Validated method for the quantification of baclofen in human plasma using solid-phase extraction and liquid chromatography–tandem mass spectrometry
T2 - Journal of Analytical Toxicology
UR - http://dx.doi.org/10.1093/jat/bkv125
UR - http://hdl.handle.net/10044/1/58494
VL - 40
ER -
Toxicology Unit
Imperial College London
Charing Cross Hospital Campus
St. Dunstan’s Road
London W6 8RP
+44 (0)20 3311 7108
s.paterson@imperial.ac.uk