@article{Berrones:2023:10.1002/ange.202310402,
author = {Berrones, Reyes J and Sherin, PS and Sarkar, A and Kuimova, MK and Vilar, R},
doi = {10.1002/ange.202310402},
journal = {Angewandte Chemie},
pages = {e202310402--e202310402},
title = {Platinum(II)-based optical probes for imaging quadruplex DNA structures via phosphorescence lifetime imaging microscopy},
url = {http://dx.doi.org/10.1002/ange.202310402},
volume = {135},
year = {2023}
}

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TY  - JOUR
AB  - G-quadruplex DNA is a non-canonical structure that forms in guanine-rich regions of the genome. There is increasing evidence showing that G-quadruplexes have important biological functions, and therefore molecular tools to visualise these structures are important. Herein we report on a series of new cyclometallated platinum(II) complexes which, upon binding to G-quadruplex DNA, display an increase in their phosphorescence, acting as switch-on probes. More importantly, upon binding to G-quadruplexes they display a selective and distinct lengthening of their emission lifetime. We show that this effect can be used to selectively visualise these structures in cells using Phosphorescence Lifetime Imaging Microscopy (PLIM).
AU  - Berrones,Reyes J
AU  - Sherin,PS
AU  - Sarkar,A
AU  - Kuimova,MK
AU  - Vilar,R
DO  - 10.1002/ange.202310402
EP  - 202310402
PY  - 2023///
SN  - 0044-8249
SP  - 202310402
TI  - Platinum(II)-based optical probes for imaging quadruplex DNA structures via phosphorescence lifetime imaging microscopy
T2  - Angewandte Chemie
UR  - http://dx.doi.org/10.1002/ange.202310402
UR  - https://www.ncbi.nlm.nih.gov/pubmed/38516271
UR  - https://onlinelibrary.wiley.com/doi/10.1002/ange.202310402
UR  - http://hdl.handle.net/10044/1/113427
VL  - 135
ER  - 

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