@article{Bentaleb:2017:10.1186/s12879-017-2666-4,
author = {Bentaleb, EM and El, Messaoudi MD and Abid, M and Messaoudi, M and Yetisen, AK and Sefrioui, H and Amzazi, S and Benhassou, HA},
doi = {10.1186/s12879-017-2666-4},
journal = {BMC Infectious Diseases},
title = {Plasmid-based high-resolution melting analysis for accurate detection of rpoB mutations in Mycobacterium tuberculosis isolates from Moroccan patients},
url = {http://dx.doi.org/10.1186/s12879-017-2666-4},
volume = {17},
year = {2017}
}

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TY  - JOUR
AB  - BackgroundRapid diagnosis of drug resistance in tuberculosis (TB) is pivotal for the timely initiation of effective antibiotic treatment to prevent the spread of drug-resistant strains. The development of low-cost, rapid and robust methods for drug-resistant TB detection is highly desirable for resource-limited settings.MethodsWe report the use of an in house plasmid-based quantitative polymerase chain reaction-high-resolution melting (qPCR-HRM) analysis for the detection of mutations related to rifampicin-resistant Mycobacterium tuberculosis (MTB) in clinical isolates from Moroccan patients. Five recombinant plasmids containing predominant mutations (S531L, S531W, H526Y and D516V) and the wild-type sequence of the Rifampicin Resistance-Determining Region (RRDR) have been used as controls to screen 45 rifampicin-resistant and 22 rifampicin-susceptible MTB isolates.ResultsThe sensitivity and the specificity of the qPCR-HRM analysis were 88.8% and 100% respectively as compared to rifampicin Drug Susceptibility Testing (DST). The results of qPCR-HRM and DNA sequencing had a concordance of 100%.ConclusionOur qPCR-HRM assay is a sensitive, accurate and cost-effective assay for the high-throughput screening of mutation-based drug resistance in TB reference laboratories.
AU  - Bentaleb,EM
AU  - El,Messaoudi MD
AU  - Abid,M
AU  - Messaoudi,M
AU  - Yetisen,AK
AU  - Sefrioui,H
AU  - Amzazi,S
AU  - Benhassou,HA
DO  - 10.1186/s12879-017-2666-4
PY  - 2017///
SN  - 1471-2334
TI  - Plasmid-based high-resolution melting analysis for accurate detection of rpoB mutations in Mycobacterium tuberculosis isolates from Moroccan patients
T2  - BMC Infectious Diseases
UR  - http://dx.doi.org/10.1186/s12879-017-2666-4
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000407072100003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://bmcinfectdis.biomedcentral.com/articles/10.1186/s12879-017-2666-4
UR  - http://hdl.handle.net/10044/1/96789
VL  - 17
ER  - 

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