Super Resolution TIRF Microscope

Photograph of the super resolution microscope in the lab

Nikon RF super-resolution microscope in the lab. Photo by Michael Morten

Researcher using the super resolution microscope in the lab

Photo by Thomas Angus

Fluorescent super resolved image of neuronal cells

iPSC Derived Neural Stem Cells, stained for Nestin (Green) and Pax6 (Red). Image captured using the TIRF microscope. Credit: Ye lab

Technical experts

Dr Yu Ye
UK DRI Group Leader

Dr Michael Morten
Postdoctoral researcher - Ye lab

Ziba Shadman
Research Technician - Ye lab

Location: Level 7 Sir Michael Uren Hub, White City Campus
Technique: Microscopy - Light
Manufacturer: Nikon
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The Nikon Ti2 inverted fluorescence microscope provides a motorized and intelligent platform for advanced imaging applications.

The TIRF microscope increases the signal-to-background ratio compared to traditional epi-microscopy and facilitates imaging at single-molecule resolutions (~ 25 nm). Imaging below the diffraction limit enables the characterisation of the structure of protein complexes inside cells and tissue samples. The EM-CCD camera can record movies at 1 frame/ms, and this setup can perform single-particle tracking using multiple colours in live cells at high speeds. These techniques can be controlled through custom written scripts which enables automatic data collection and increases the microscope’s capacity for high-throughput imaging.

Contact

For enquiries regarding the technology, please contact Dr Michael Morten, a postdoctoral researcher in the Ye lab.
To discuss use of the microscope, please contact Dr Yu Ye, UK DRI Group Leader.

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