Hands-on Data Analysis for Metabolic Profiling

By the end of the course you will be able to:

• Describe the main ideas and concepts underlying untargeted metabolomics
• Outline the characteristics of NMR & LCMS metabolomic data, and how these influence data analysis
• Process both NMR and LCMS metabolomic data, ensuring high quality and reproducibility
• Discuss the concepts, strengths and weaknesses of multivariate statistical techniques such as PCA and OPLS as applied to metabolomics data
• Summarise the main data-driven techniques helping to identify unknown metabolites
• Explain the main ideas behind pathway analysis with metabolite data
• Apply all these tools to your own data with confidence and understanding

Learning and teaching methods

The course consists of:

• Lectures covering the computational metabolomics techniques
• Hands-on practical sessions analysing example data sets
• Discussion and Q&A sessions addressing key data analysis topics

We offer a unique course in metabolomics data analysis, blending lectures and practical computational experience. Small attendee numbers ensure that there is maximum opportunity to interact with experienced instructors. On completion of the course, attendees will understand the aims of untargeted metabolomics and the characteristics of metabolomic data from NMR and LC-MS and have a good understanding of pre-processing and statistical modelling of the data. A key benefit is training in the use of open source software which attendees can use immediately on their own data.

Our courses are designed for customers from universities, research institutions and industry who wish to expand their knowledge and skills in metabolomics, including:

• Analytical scientists working with complex mixtures
• Bioinformaticians or statisticians wishing to understand metabolomics techniques
• Clinicians interested in applying metabolomics to their clinical studies

The course assumes no prior knowledge of multivariate statistics, and does not require prior coding experience.  To fully benefit from this course, attendees will ideally have a basic knowledge of analytic chemistry techniques.

All participants will be awarded an Imperial College London Certificate of Attendance on completion of the course.

This course has been awarded 20 CPD credits by the Royal Society of Medicine in accordance with its current guidelines.