Microscopic image of mitochondriaThis page contains a list of useful web links and documents relating to sample preparation, fluorophores (dyes) and other imaging requirements

Resources

General information
Spectral information on fluorophores, filters and lamps
Deconvolution
  • Nyquist Calculator - calculates the optimal sampling rate for microscopic image acquisition (Nyquist rate = 2x maximal resolution) Scientific Volume Imaging
Information on objectives
General imaging and analysis software

Tutorial: guidance for quantitative confocal microscopy by James Jonkman, Claire M. Brown, Graham D. Wright, Kurt I. Anderson and Alison J. North. Nature Protocols 2020

See our Software Pages - Software

 

Whole figure preparation
Protocols, tips and tricks - sample preparation

This is a growing collection of methods and techniques used in the facility, representing the enormous range of expertise of our microscopists. Everybody is very much invited to contribute with their own protocols. Protocols are provided as Word files, so they can be downloaded and edited as required.

Presentations

General enquiries


FILM
Sir Alexander Fleming Building
South Kensington Campus
Imperial College London
Exhibition Road
London SW7 2AZ, UK

film-service@imperial.ac.uk